Description

Formononetin3Monooxygenase is a specialized enzyme involved in the biosynthesis of isoflavonoids, a class of compounds with significant biological activity. This enzyme plays a critical role in catalyzing specific hydroxylation reactions, making it a valuable tool for biochemical research and the development of bioactive compounds. It is essential for researchers and manufacturers in the pharmaceutical, nutraceutical, and agricultural biotechnology sectors who are exploring plant metabolism, enzyme mechanisms, and the production of high-value isoflavonoids.

Application

• Biochemical Research: Used as a key reagent in studies of flavonoid and isoflavonoid biosynthetic pathways.
• Enzyme Mechanism Studies: Employed to investigate catalytic activity, substrate specificity, and inhibition kinetics of monooxygenase enzymes.
• Pharmaceutical Development: Serves as a tool for the biocatalytic synthesis of potential drug intermediates or active pharmaceutical ingredients (APIs) based on isoflavonoid scaffolds.
• Nutraceutical Production: Utilized in research aimed at enhancing the yield or modifying the profile of bioactive isoflavones in functional foods and supplements.
• Agricultural Biotechnology: Applied in genetic engineering projects to modify plant secondary metabolism for improved stress resistance or nutritional content.
• Reference Standard: Acts as a purified enzyme standard for analytical method development and validation in quality control laboratories.

Basic Information

Product Name	Formononetin3Monooxygenase
CAS No.	133249-59-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Formononetin 3'-Monooxygenase; CYP81E7 (common cytochrome P450 designation); Isoflavone 3'-hydroxylase; 2-Hydroxyformononetin synthase; [NADPH:oxygen] oxidoreductase (formononetin 3'-hydroxylating); P450 81E7
EINECS	Contact for details

Quality Control

Our Formononetin3Monooxygenase is produced and handled under stringent quality management protocols. Each batch undergoes rigorous analytical testing to ensure high purity, specific activity, and functional integrity. Certificates of Analysis (COA) detailing purity, activity units, and stability data are available upon request to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This material is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after use to minimize exposure to atmospheric moisture. For short-term use, keep aliquots at 2-8°C.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Purity (SDS-PAGE)	≥ 90%
Specific Activity	Defined units/mg protein
Protein Concentration	Defined mg/mL
Buffer Composition	Defined buffer, pH, stabilizers
Endotoxin Level	< 1.0 EU/μg (if applicable)
Biological Activity	Confirmed by enzymatic assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.