Description

Formononetin 2-Monooxygenase is a specialized enzyme belonging to the cytochrome P450 family, identified by CAS No. 133249-58-8. This biocatalyst is critical for the selective hydroxylation of isoflavonoids, enabling the synthesis of high-value intermediates for pharmaceutical and nutraceutical research. It is an essential tool for biotechnology firms, academic research institutions, and manufacturers focused on developing novel therapeutics, plant-based bioactive compounds, and advanced biochemical pathways.

Application

• Biocatalysis & Enzymatic Synthesis: Key catalyst for the regioselective hydroxylation of formononetin and related isoflavones in green chemistry processes.
• Pharmaceutical Intermediate Production: Used in the biosynthesis of daidzein and other pharmacologically active isoflavonoid derivatives for drug development.
• Nutraceutical & Functional Food Research: Facilitates the production of standardized, bioactive isoflavone extracts with enhanced properties.
• Metabolic Pathway Engineering: A vital component in constructing microbial cell factories for the sustainable production of plant-specialized metabolites.
• Biochemical & Enzymology Studies: Serves as a model enzyme for studying cytochrome P450 reaction mechanisms, kinetics, and substrate specificity.
• Agricultural Biotechnology: Potential use in developing crops with modified isoflavonoid profiles for improved stress resistance or nutritional value.

Basic Information

Item	Details
Product Name	Formononetin 2-Monooxygenase
CAS No.	133249-58-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Formononetin 2'-Hydroxylase; CYP81E7 (in specific plant species); Isoflavone 2'-Hydroxylase; Cytochrome P450 81E7; Formononetin,NADPH:oxygen oxidoreductase (2'-hydroxylating); P450 monooxygenase (Formononetin); NADPH-Ferrihemoprotein Reductase (Formononetin 2'-Monooxygenase)
EINECS	Contact for details

Quality Control

Our enzyme preparations are produced and tested under stringent quality management systems. Each batch undergoes comprehensive analysis to ensure high specific activity, purity, and stability. Quality is verified through assays for protein content, enzymatic activity, and the absence of contaminating protease or nuclease activities. A Certificate of Analysis (COA) detailing batch-specific performance data is provided with every shipment to support your research and quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below in aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use. Handle under appropriate laboratory conditions.

Specification

Item	Specification
Appearance	Lyophilized powder or clear solution
Protein Content (Bradford)	>90%
Specific Activity	≥ [Value] U/mg protein
Purity (SDS-PAGE)	Single major band
Buffer Composition	Specified phosphate or Tris buffer, pH 7.0-7.5
Stabilizers	May contain glycerol or BSA
Contaminants	Protease activity: Not detected; Nuclease activity: Not detected

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.