Description

25-Hydroxyvitamin D3 3'-(n-(4-Azido-2-Nitrophenyl)Amino)Propyl Ether is a chemically modified derivative of 25-Hydroxyvitamin D3, designed as a photoreactive affinity labeling reagent for biochemical research. This compound matters because it enables the covalent cross-linking and identification of vitamin D receptor (VDR) binding proteins and other molecular targets, providing critical insights into metabolic pathways. It is primarily needed by research institutions, pharmaceutical R&D departments, and diagnostic kit manufacturers focused on endocrinology, bone metabolism, and cancer research.

Application

• Photoreactive Affinity Labeling: Used as a key reagent for photoaffinity labeling studies to investigate the binding sites and interactions of the vitamin D receptor (VDR) and its metabolites.
• Biochemical Probe Development: Serves as a critical intermediate in the synthesis of probes for studying vitamin D metabolism, transport, and signaling mechanisms.
• Drug Discovery Research: Employed in pharmaceutical R&D for target identification and validation in programs related to osteoporosis, psoriasis, and metabolic disorders.
• Diagnostic Assay Development: Utilized in the development and calibration of competitive binding assays (e.g., ELISA, RIA) for the quantitative detection of vitamin D metabolites.
• Metabolic Pathway Analysis: Applied in academic and industrial research to trace and elucidate the complex metabolic pathways of vitamin D and its analogs.
• Protein Interaction Studies: Functions as a tool compound for pull-down assays and mass spectrometry to identify novel proteins that interact with vitamin D and its derivatives.

Basic Information

Product Name	25-Hydroxyvitamin D3 3'-(n-(4-Azido-2-Nitrophenyl)Amino)Propyl Ether
CAS No.	133191-08-9
Molecular Formula	C33H49N5O4
Molecular Weight	579.78 g/mol
Synonyms	25-Hydroxyvitamin D3 3'-(NAP) Propyl Ether; 25(OH)D3-3'-NAP Ether; 25-Hydroxycholecalciferol 3'-(4-Azido-2-Nitrophenylamino)Propyl Ether; Vitamin D3 Metabolite Affinity Label; 25-Hydroxyvitamin D3 Photoaffinity Label; 25(OH)D3 Photoprobe; 1α,25-Dihydroxyvitamin D3 Precursor Derivative; 3'-(N-(4-Azido-2-nitrophenyl)amino)propyl Ether of 25-Hydroxyvitamin D3
EINECS	Contact for details

Quality Control

Our 25-Hydroxyvitamin D3 3'-(n-(4-Azido-2-Nitrophenyl)Amino)Propyl Ether is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency for research applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity and identity confirmation via NMR and mass spectrometry. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation. The container should be kept in a dry, cool, and well-ventilated area. Allow the product to equilibrate to room temperature in the original, unopened container before use to minimize condensation and degradation.

Specification

Item	Specification
Appearance	Yellow to orange powder or solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95%
Related Substances (HPLC)	Total impurities ≤ 5.0%
Solubility	Soluble in DMSO, ethanol, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.