Description

Tup1 Protein CAS NO 133135-32-7 is a highly purified recombinant protein, a key component of the SAGA/ADA chromatin remodeling complex involved in transcriptional regulation. This product is essential for researchers and developers in the life sciences sector requiring consistent, high-quality biological reagents for mechanistic studies. It is primarily utilized in biochemical research, drug discovery, and molecular biology applications focusing on gene expression and epigenetic mechanisms.

Application

• Biochemical Research: Used as a critical reagent for studying the structure and function of the SAGA/ADA coactivator complex and its role in histone acetylation.
• Drug Discovery & Screening: Serves as a target protein in assays designed to identify novel therapeutics that modulate chromatin remodeling and transcriptional activity.
• Protein-Protein Interaction Studies: Employed in pull-down assays, co-immunoprecipitation (Co-IP), and yeast two-hybrid systems to map interaction networks within transcriptional machinery.
• Enzymology Studies: Used in in vitro assays to investigate the enzymatic activity of associated complexes, such as histone acetyltransferase (HAT) assays.
• Antigen Production: Acts as an immunogen for generating specific antibodies used in Western blotting, immunofluorescence, and ChIP-seq experiments.
• Molecular Biology Reagent: A component in reconstituted systems for in vitro transcription and chromatin remodeling assays.

Basic Information

Item	Detail
Product Name	Tup1 Protein
CAS No.	133135-32-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Tup1; General repressor protein TUP1; Transcriptional regulator TUP1; Tup1p; S. cerevisiae Tup1 protein; Chromatin remodeling complex subunit TUP1; Yeast Tup1 Protein; Recombinant Tup1 Protein
EINECS	Contact for details

Quality Control

Our Tup1 Protein is produced under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous quality testing, including purity analysis by SDS-PAGE and HPLC, functional activity assays, and endotoxin level checks. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and activity is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Upon reconstitution, aliquot and store at -80°C to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the vial is equilibrated to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White or off-white lyophilized powder
Identification (SDS-PAGE)	Single band corresponding to expected molecular weight
Purity (HPLC)	≥ 95%
Protein Content	Determined by UV absorbance or Bradford assay
Biological Activity	Confirmed by functional assay (e.g., binding assay)
Endotoxin Level	< 1.0 EU/µg (for cell culture applications)
Solubility	Soluble in appropriate aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.