Description

Let-60 Protein CAS NO 133135-08-7 is a recombinant protein derived from the *C. elegans* Let-60 gene, a homolog of the mammalian Ras oncoprotein. This product is essential for researchers studying signal transduction pathways, cellular differentiation, and oncogenesis. It is a critical reagent for biochemical assays, protein-protein interaction studies, and functional genomics in the life sciences and pharmaceutical R&D sectors.

Application

• Biochemical Research: Used as a substrate or binding partner in enzymatic activity assays to study GTPase function and regulation.
• Signal Transduction Studies: Essential for investigating the Ras/MAPK pathway, a key regulator of cell growth, proliferation, and differentiation.
• Drug Discovery & Screening: Serves as a target protein in high-throughput screening (HTS) platforms to identify novel inhibitors or modulators of Ras-like proteins.
• Protein-Protein Interaction Analysis: Utilized in pull-down assays, co-immunoprecipitation (Co-IP), and surface plasmon resonance (SPR) to map interaction networks.
• Antibody Production & Validation: Acts as an immunogen for generating specific antibodies or as a standard for antibody specificity testing in Western blot and ELISA.
• Academic & Pharmaceutical R&D: A fundamental tool for basic research in developmental biology, cancer research, and functional proteomics.

Basic Information

Product Name	Let-60 Protein
CAS No.	133135-08-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Recombinant Let-60 Protein; C. elegans Ras-like Protein Let-60; GTPase Let-60; Ras-related Protein Let-60; Oncogene Protein Let-60; LET-60; CELE_K12H4.8 Gene Product
EINECS	Contact for details

Quality Control

Our Let-60 Protein is produced under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes comprehensive analysis, including purity assessment by SDS-PAGE and HPLC, identity confirmation by mass spectrometry, and functional activity testing. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use. Handle under appropriate laboratory conditions.

Specification

Item	Specification
Appearance	White or off-white lyophilized powder
Identification (Mass Spec)	Conforms to structure
Purity (SDS-PAGE)	≥ 90%
Purity (HPLC)	≥ 95%
Biological Activity	Determined by functional assay (e.g., GTP binding)
Endotoxin Level	< 1.0 EU/µg
Protein Content	Determined by UV absorbance
Solubility	Soluble in appropriate aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.