Description

Sephacryl S 400Hr is a high-resolution, cross-linked allyl dextran gel filtration chromatography medium designed for the separation of large biomolecules. This product is essential for achieving precise fractionation of proteins, polysaccharides, and other macromolecules in the 20,000 to 8,000,000 molecular weight range. It is a critical tool for downstream processing and analytical applications in the biopharmaceutical, academic research, and industrial biotechnology sectors. The CAS number for this compound is 132703-24-3.

Application

• Purification of High Molecular Weight Proteins: Ideal for separating antibodies, antibody fragments, and viral particles in biopharmaceutical manufacturing.
• Size-Exclusion Chromatography (SEC): Used for determining the molecular weight distribution of polymers, polysaccharides, and protein aggregates.
• Desalting and Buffer Exchange: Efficient removal of salts, detergents, or other small molecules from protein and nucleic acid samples.
• Fractionation of Polysaccharides and Dextrans: Critical in the food, cosmetic, and material science industries for polymer analysis and purification.
• Plasmid DNA and Large Nucleic Acid Purification: Supports downstream processing in gene therapy and vaccine development workflows.
• Process Development and Scale-Up: Offers excellent mechanical and chemical stability for reproducible results from laboratory to pilot and production scale.

Basic Information

Product Name	Sephacryl S 400Hr
CAS No.	132703-24-3
Molecular Formula	Proprietary cross-linked polymer
Molecular Weight	N/A (Chromatography medium)
Synonyms	Sephacryl S-400 HR; Sephacryl S400 High Resolution; Allyl Dextran Cross-linked with N,N'-Methylenebisacrylamide; Size Exclusion Chromatography Gel S-400; Gel Filtration Medium S 400 HR
EINECS	Contact for details

Quality Control

Every batch of Sephacryl S 400Hr is manufactured under a quality management system and undergoes rigorous testing to ensure performance consistency for critical separations. Key parameters such as particle size distribution, exclusion limits, flow rate, and pressure stability are validated. A Certificate of Analysis (COA) detailing lot-specific performance data is provided with each shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at room temperature (15-25°C) in its supplied storage solution (typically 20% ethanol) to prevent microbial growth and maintain bead integrity. Do not allow the medium to freeze. For long-term storage, ensure the container is sealed to prevent evaporation.

Specification

Item	Specification
Matrix	Cross-linked allyl dextran with N,N'-methylenebisacrylamide
Particle Size	25 - 75 μm (mean)
Separation Range (Globular Proteins)	20,000 - 8,000,000 Da
Exclusion Limit (Dextrans)	> 20,000,000 Da
Maximum Operating Pressure	0.3 MPa (3 bar, 43 psi)
pH Stability (long-term)	3 - 11
pH Stability (short-term)	2 - 13
Chemical Stability	Stable in common aqueous buffers, 1M NaOH, 8M urea, 6M guanidine HCl, and 30% isopropanol.
Storage Solution	20% Ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.