Description

A20 Protein CAS NO 131553-65-6 is a high-purity recombinant protein, also known as Tumor Necrosis Factor α-Induced Protein 3 (TNFAIP3), which plays a critical role as a negative regulator of the NF-κB signaling pathway and inflammatory responses. Its importance lies in its function as a key deubiquitinating enzyme, making it an essential research tool for studying cellular signaling, immune regulation, and apoptosis. This protein is primarily needed by researchers and developers in the fields of immunology, oncology, and molecular biology for applications ranging from basic research to drug discovery and diagnostic assay development.

Application

• Biomedical Research: Used as a critical reagent for studying the NF-κB signaling pathway, inflammatory responses, and programmed cell death (apoptosis).
• Drug Discovery & Development: Serves as a target protein for screening and validating potential therapeutic compounds aimed at modulating immune and inflammatory diseases.
• Diagnostic Assay Development: Employed in the development of ELISA kits, Western blot controls, and other immunoassays to detect and quantify related biological markers.
• Enzymatic Activity Studies: Utilized in in vitro deubiquitination assays to investigate enzyme kinetics, substrate specificity, and inhibitor effects.
• Cell Signaling & Pathway Analysis: Essential for protein-protein interaction studies and understanding the molecular mechanisms in autoimmune disorders and cancers.
• Recombinant Protein Production: Acts as a reference standard for quality control in the production of other recombinant proteins and biologics.

Basic Information

Item	Details
Product Name	A20 Protein
CAS No.	131553-65-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Tumor Necrosis Factor α-Induced Protein 3 (TNFAIP3); A20 Tumor Necrosis Factor α-Induced Protein 3; OTU Domain-Containing Protein 7C (OTUD7C); Zinc Finger Protein A20; TNF α-Induced Protein 3; Putative DNA-binding Protein A20; A20
EINECS	Contact for details

Quality Control

Our A20 Protein is produced under stringent conditions to ensure the highest levels of purity and biological activity. Each batch undergoes rigorous analytical testing, including SDS-PAGE, HPLC, and functional activity assays, to confirm identity, purity, and consistency. Certificates of Analysis (COA) detailing specific results for each lot are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. Avoid repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear solution
Identification (SDS-PAGE)	Single band at expected molecular weight
Purity (HPLC)	≥ 95%
Protein Content	Determined by UV absorbance or Bradford assay
Biological Activity	Determined by functional deubiquitination assay
Endotoxin Level	< 1.0 EU/µg (for cell culture grades)
Solubility	Soluble in appropriate aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.