Description

1-(n-(2-Hydroxy-5-Azidobenzoyl)-2-Aminoethyl)-4-(n-Hydroxysuccinimidyl)Succinate is a specialized heterobifunctional crosslinking reagent designed for advanced bioconjugation. This compound is engineered to facilitate the stable and site-specific coupling of biomolecules, such as proteins and peptides, to various surfaces or other molecules. It is an essential tool for researchers and developers in the fields of pharmaceutical development, diagnostic assay manufacturing, and advanced materials science, where precise and reliable molecular attachment is critical.

Application

• Bioconjugation and Crosslinking: Primary reagent for covalently linking proteins, antibodies, or peptides to nanoparticles, polymers, or solid supports for diagnostic and therapeutic platforms.
• Diagnostic Kit Manufacturing: Used in the development of enzyme-linked immunosorbent assays (ELISA), lateral flow assays, and other immunoassays to immobilize capture antibodies.
• Drug Delivery System Development: Facilitates the attachment of targeting ligands or therapeutic payloads to carrier systems like liposomes or polymeric nanoparticles.
• Surface Functionalization: Enables the modification of biosensor surfaces, microarray slides, and medical device coatings with bioactive molecules.
• Affinity Chromatography Resin Production: Key component in creating ligand-coupled resins for the purification of specific biomolecules.
• Proteomics and Chemical Biology Research: Employed in studying protein-protein interactions, protein labeling, and the construction of biomolecular complexes.

Basic Information

Item	Detail
Product Name	1-(n-(2-Hydroxy-5-Azidobenzoyl)-2-Aminoethyl)-4-(n-Hydroxysuccinimidyl)Succinate
CAS No.	131483-43-7
Molecular Formula	C21H20N6O9
Molecular Weight	500.42 g/mol
Synonyms	NHS-Azide Crosslinker; 2-Hydroxy-5-azidobenzoic acid N-hydroxysuccinimide ester derivative; Heterobifunctional crosslinker with NHS ester and aryl azide; 5-Azido-2-hydroxybenzoyl-PEG2-NHS ester; Aryl azide-PEG2-NHS ester; Succinimidyl 4-((2-((2-hydroxy-5-azidobenzoyl)amino)ethyl)amino)-4-oxobutanoate; SASD (Succinimidyl 2-((2-((2-hydroxy-5-azidobenzoyl)amino)ethyl)amino)-2-oxoacetate) analog
EINECS	Contact for details

Quality Control

Our production of this high-purity crosslinker adheres to stringent quality protocols. Every batch is characterized using advanced analytical techniques to ensure consistent reactivity and purity, critical for reproducible bioconjugation results. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specifications for identity, purity, and functional performance. We support development under ISO 9001 quality management systems, and our manufacturing processes are designed to meet the exacting standards required for research and in-vitro diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store desiccated under inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.