Description

1-Oleoyl-2-(12-((7-Nitro-2,1,3-Benzoxadiazol-4-Yl)Amino)Dodecanoyl)Phosphatidylcholine is a highly specialized, fluorescently labeled phospholipid derivative, crucial for advanced membrane biophysics and cell biology research. Its core value lies in the covalent attachment of the NBD (7-nitrobenz-2-oxa-1,3-diazol-4-yl) fluorophore, enabling real-time visualization and quantitative analysis of lipid dynamics, trafficking, and membrane fusion events. This compound is essential for researchers and formulators in pharmaceutical development, diagnostic assay design, and advanced biomaterials science who require precise, sensitive probes to study complex biological systems.

Application

• Fluorescent Biomembrane Probe: Used to label and trace the behavior of phosphatidylcholine in artificial lipid bilayers (liposomes) and cellular membranes.
• Lipid Trafficking & Metabolism Studies: Enables visualization of intracellular lipid transport, sorting, and metabolic pathways in live-cell imaging.
• Membrane Fusion Assays: Serves as a key component in fluorescence resonance energy transfer (FRET) or dequenching assays to monitor vesicle fusion kinetics.
• Drug Delivery System Research: Incorporated into liposomal formulations to study carrier stability, cellular uptake mechanisms, and intracellular fate.
• Lipoprotein & Lipid Particle Analysis: Utilized as a fluorescent tag to investigate the structure and remodeling of high-density lipoproteins (HDL) and other lipid-protein complexes.
• Enzymology Studies: Acts as a substrate or inhibitor for phospholipases (e.g., PLA2) and other lipid-modifying enzymes, allowing fluorometric activity measurement.

Basic Information

Product Name	1-Oleoyl-2-(12-((7-Nitro-2,1,3-Benzoxadiazol-4-Yl)Amino)Dodecanoyl)Phosphatidylcholine
CAS No.	131479-28-2
Molecular Formula	C53H90N5O12P
Molecular Weight	1020.28 g/mol
Synonyms	NBD-PC; 1-Oleoyl-2-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoyl]-sn-glycero-3-phosphocholine; NBD C12-HPC; Fluorescent Phosphatidylcholine, NBD labeled; 2-(NBD-amino)dodecanoyl-1-oleoyl-sn-glycero-3-phosphocholine; NBD-PtdCho; 1-Oleoyl-2-(NBD-dodecanoyl)-PC
EINECS	Contact for details

Quality Control

Every batch of our fluorescent lipids is produced and analyzed under strict quality control protocols to ensure high purity and performance consistency for sensitive research applications. We employ advanced chromatographic (HPLC) and spectroscopic techniques for identity confirmation and impurity profiling. A comprehensive Certificate of Analysis (COA) detailing purity, fluorescence properties, and solvent content is provided with each shipment to support your experimental reproducibility and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below under an inert atmosphere (argon or nitrogen). This compound is strictly light-sensitive (protect from all light exposure) and easily oxidized (store under inert atmosphere). Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 98% (Area Percent)
Solvent Content (Residual Chloroform/Methanol)	≤ 1.0% (by 1H-NMR or GC)
Fluorescence Properties (Ex/Em)	~465 nm / ~535 nm in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.