Description

Cholera Toxin B Subunit CAS NO 131096-89-4 is a high-purity, non-toxic protein subunit derived from the cholera toxin, widely recognized for its specific binding affinity to GM1 gangliosides on cell surfaces. This makes it an invaluable tool in life science research for studying cell signaling pathways, membrane trafficking, and as a neuronal tracer. It is essential for researchers and manufacturers in the fields of immunology, neuroscience, vaccine development, and diagnostic assay production.

Application

• Neuroscience Research: Used as a highly sensitive retrograde tracer to map neuronal pathways due to its specific uptake and transport mechanisms.
• Immunology & Vaccine Development: Serves as a potent mucosal immunogen and adjuvant carrier to enhance immune responses in vaccine formulations.
• Cell Biology Studies: Employed to investigate endocytosis, intracellular transport, and lipid raft dynamics via its GM1 ganglioside binding property.
• Diagnostic Reagents: Acts as a key component in ELISA and other immunoassays for the detection of specific antibodies or cellular markers.
• Drug Delivery Research: Utilized as a targeting moiety to deliver therapeutic agents specifically to cells expressing GM1.
• Quality Control in Biopharma: Applied in the testing and validation of processes for related biopharmaceutical products.

Basic Information

Product Name	Cholera Toxin B Subunit
CAS No.	131096-89-4
Molecular Formula	C~580H~900N~152O~168S~6 (Approximate for pentamer)
Molecular Weight	~58 kDa (Pentamer)
Synonyms	Cholera Toxin Subunit B; CTB; Choleragenoid; Cholera Enterotoxin B Subunit; Vibrio cholerae Toxin B Subunit; GM1 Ganglioside Binding Protein; Cholera Toxin B Pentamer; Cholera Toxin (Biotinylated); Recombinant Cholera Toxin B Subunit
EINECS	Contact for details

Quality Control

Our Cholera Toxin B Subunit is produced and tested under stringent conditions to ensure the highest standards of purity and biological activity, suitable for research and in vitro diagnostic use. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing key parameters such as purity, endotoxin levels, and binding activity. We adhere to ISO 9001 quality management principles, and our processes are designed to support compliance with relevant regulatory frameworks for research materials.

Storage

Preserve in a tightly closed container, protected from light. Store lyophilized material at -20°C or below. For reconstituted solutions, aliquot and store at -70°C to -80°C to prevent repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after each use in a low-humidity environment.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (SDS-PAGE)	Corresponds to standard
Purity (HPLC)	≥ 95%
Protein Content (UV)	≥ 90% of dry weight
Biological Activity (GM1 Binding)	Confirmed
Endotoxin Level (LAL)	< 1.0 EU/µg
Solubility	Soluble in aqueous buffers
Moisture Content (KF)	< 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.