Description

5-Bromo-4-Chloro-3-Indoxyl-β-D-Mannopyranoside is a specialized chromogenic substrate used for the enzymatic detection of β-mannosidase activity. This compound is critical for research and diagnostic applications where precise, colorimetric visualization of enzymatic processes is required. It is primarily utilized by biochemists, molecular biologists, and diagnostic kit manufacturers in life science research, microbiology, and clinical diagnostics.

Application

• Enzymatic Substrate for β-Mannosidase: Used as a specific chromogenic substrate in assays to detect and quantify β-mannosidase enzyme activity.
• Microbiological Research: Employed in microbial identification and characterization studies to differentiate bacterial strains based on their enzymatic profiles.
• Diagnostic Kit Manufacturing: A key component in the formulation of diagnostic kits and reagents for clinical and research laboratories.
• Biochemical Pathway Analysis: Facilitates the study of glycoside hydrolase functions and carbohydrate metabolism in various biological systems.
• Cell and Tissue Staining: Used in histochemistry for the localization of β-mannosidase activity in tissue sections and cell cultures.
• Quality Control in Biotechnology: Applied in the quality assurance of enzyme preparations and recombinant protein products.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indoxyl-β-D-Mannopyranoside
CAS No.	129787-67-3
Molecular Formula	C14H15BrClNO7
Molecular Weight	424.63 g/mol
Synonyms	5-Bromo-4-chloro-3-indolyl-β-D-mannopyranoside; X-β-Man; BCIP-β-Man; 5-Br-4-Cl-3-indoxyl-β-D-mannoside; β-Mannosidase Substrate; Chromogenic β-Mannosidase Substrate; 3-Indolyl-β-D-mannopyranoside, 5-bromo-4-chloro derivative
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indoxyl-β-D-Mannopyranoside is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot is analyzed via HPLC, NMR, and mass spectrometry to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the container to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.