Description

Fluorescein Mono-β-D-Galactopyranoside is a specialized fluorogenic substrate designed for high-sensitivity enzymatic detection. This compound is critical for research and diagnostic applications requiring precise measurement of β-galactosidase activity. It is primarily utilized by biotechnology, pharmaceutical, and diagnostic reagent manufacturers for applications in cell-based assays, reporter gene studies, and enzyme-linked detection systems.

Application

• Reporter Gene Assays: A key substrate for detecting β-galactosidase expression in molecular and cellular biology research.
• Diagnostic Kits: Used in the development of fluorometric diagnostic tests and immunoassays for clinical and research use.
• High-Throughput Screening (HTS): Enables sensitive detection of enzymatic activity in drug discovery and biochemical screening platforms.
• Cell Viability & Proliferation Assays: Employed in assays to monitor cell health and gene expression dynamics.
• Enzyme Kinetics Studies: Serves as a specific substrate for studying the kinetics and inhibition of β-galactosidase enzymes.
• Microbiology Research: Used in bacterial detection systems that utilize β-galactosidase as a marker.

Basic Information

Product Name	Fluorescein Mono-β-D-Galactopyranoside
CAS No.	129787-63-9
Molecular Formula	C25H28O13
Molecular Weight	536.48 g/mol
Synonyms	Fluorescein-β-D-Galactopyranoside; FMG; Fluorescein mono-β-D-galactoside; 5(6)-Carboxyfluorescein di-β-D-galactopyranoside (related); β-Gal-Fluor; Fluorogenic β-galactosidase substrate; FDG (Fluorescein Di-β-D-Galactopyranoside) analog; 3',6'-Dihydroxyfluoran-β-D-galactopyranoside
EINECS	Contact for details

Quality Control

Our Fluorescein Mono-β-D-Galactopyranoside is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and performance.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Fluorogenic Activity	Functional in standard β-galactosidase assay
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.