Description

L-Fmoc-Aspartic Acid α-tert-Butyl Ester is a crucial, high-purity protected amino acid derivative essential for modern solid-phase peptide synthesis (SPPS). Its primary value lies in providing orthogonal protection for the side-chain carboxylic acid of aspartic acid, enabling the controlled synthesis of complex peptides and proteins. This compound is indispensable for researchers and manufacturers in the pharmaceutical, biotechnology, and life sciences sectors, particularly in the development of therapeutic peptides, peptide hormones, and biochemical research reagents.

Application

• Solid-Phase Peptide Synthesis (SPPS): A key building block for the controlled, sequential assembly of peptides, especially those containing aspartic acid residues.
• Pharmaceutical Intermediates: Used in the research and production of active pharmaceutical ingredients (APIs) for peptide-based drugs.
• Bioconjugation and Protein Engineering: Facilitates the site-specific modification of proteins and the creation of peptide-protein hybrids.
• Combinatorial Chemistry Libraries: Serves as a versatile monomer for generating diverse peptide libraries for drug discovery and screening.
• Biochemical Research: Employed in academic and industrial labs to study protein structure, function, and interactions.
• Protecting Group Strategy: The Fmoc (9-fluorenylmethoxycarbonyl) and tert-butyl ester groups allow for selective deprotection under mild conditions, critical for synthesizing sensitive peptides.

Basic Information

Product Name	L-Fmoc-Aspartic Acid α-tert-Butyl Ester
CAS No.	129460-09-9
Molecular Formula	C23H25NO6
Molecular Weight	411.45 g/mol
Synonyms	Fmoc-Asp(OtBu)-OH; N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-L-aspartic acid 1-tert-butyl ester; (2S)-2-[(9H-Fluoren-9-ylmethoxy)carbonylamino]butanedioic acid 1-tert-butyl ester; Fmoc-L-Asp(OtBu)-OH; N-α-Fmoc-L-aspartic acid α-tert-butyl ester; N-Fmoc-L-aspartic acid 1-tert-butyl ester; L-Aspartic acid, N-[(9H-fluoren-9-ylmethoxy)carbonyl]-, 1-(1,1-dimethylethyl) ester
EINECS	Contact for details

Quality Control

Our L-Fmoc-Aspartic Acid α-tert-Butyl Ester is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research and cGMP applications. Each lot is subjected to rigorous analytical testing including HPLC, NMR, and optical rotation to confirm identity, purity, and chiral integrity. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This material is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions should be avoided to maintain stability and purity. Keep container tightly sealed under an inert atmosphere when not in use.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥98.5%
Optical Rotation [α]20/D	-15.0° to -18.0° (c=1 in DMF)
Water Content (KF)	≤0.5%
Heavy Metals	≤10 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.