Description

Digoxigenin NHS Ester is a high-purity, activated chemical derivative of digoxigenin, specifically designed for efficient covalent conjugation to primary amines. This compound is critical for creating stable, non-radioactive labels for sensitive detection in molecular biology and diagnostic applications. It is an essential reagent for researchers and manufacturers in the fields of immunoassay development, nucleic acid hybridization, and protein labeling.

Application

• Immunoassay Development: Conjugation to antibodies or proteins for use in ELISA, Western blotting, and immunohistochemistry.
• Nucleic Acid Labeling: Preparation of digoxigenin-labeled DNA or RNA probes for in situ hybridization (ISH) and blotting techniques.
• Diagnostic Kit Manufacturing: A key component in the production of commercial diagnostic test kits for clinical and research laboratories.
• Protein Tagging and Detection: Covalent attachment to peptides or proteins for purification, tracking, or interaction studies.
• Cell Biology Research: Used in fluorescence and chromogenic detection systems for visualizing cellular and subcellular targets.
• Biosensor Fabrication: Immobilization of biomolecules on sensor surfaces for diagnostic and analytical devices.

Basic Information

Product Name	Digoxigenin NHS Ester
CAS No.	129273-26-3
Molecular Formula	C35H48N2O10
Molecular Weight	656.77 g/mol
Synonyms	Digoxigenin 3-O-succinyl-ε-aminocaproic acid N-hydroxysuccinimide ester; Digoxigenin-3-O-methylcarbonyl-ε-aminocaproic acid N-hydroxysuccinimide ester; Digoxigenin-3-O-succinyl-ε-aminocaproic acid NHS ester; Digoxigenin succinyl-ε-aminocaproic acid NHS ester; DIG-NHS; N-Hydroxysuccinimide ester of digoxigenin-3-O-methylcarbonyl-ε-aminocaproic acid; 3β,12β,14-Trihydroxy-5β-card-20(22)-enolide 3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexanoate]
EINECS	Contact for details

Quality Control

Our Digoxigenin NHS Ester is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance. Each lot undergoes rigorous analytical testing, including HPLC for purity assessment and identity confirmation by spectroscopic methods. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and related substances is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.