Description

4-(3-(Trifluoromethyl)-3H-Diazirin-3-Yl)Benzonitrile is a specialized photolabile crosslinking agent containing a trifluoromethyl diazirine functional group. This compound is critical for advanced photoaffinity labeling (PAL) studies, enabling the covalent capture and identification of transient or weak biomolecular interactions. It is an essential research chemical for scientists in pharmaceutical discovery, chemical biology, and proteomics who require high-purity, reliable reagents for mapping protein-ligand binding sites and protein-protein interactions.

Application

• Photoaffinity Labeling (PAL) Probe Synthesis: Serves as a key precursor for creating photoactivatable probes to study biomolecular interactions.
• Chemical Biology Research: Used to develop crosslinkers for identifying drug targets and mapping binding sites in live cells.
• Proteomics and Structural Biology: Enables the covalent capture of protein complexes for mass spectrometry analysis and structural elucidation.
• Pharmaceutical R&D: Integral to fragment-based drug discovery (FBDD) and activity-based protein profiling (ABPP) for validating novel therapeutic targets.
• Material Science Functionalization: Potential use in surface modification and polymer chemistry to introduce photoreactive groups.

Basic Information

Product Name	4-(3-(Trifluoromethyl)-3H-Diazirin-3-Yl)Benzonitrile
CAS No.	128886-91-9
Molecular Formula	C9H4F3N3
Molecular Weight	211.14 g/mol
Synonyms	3-[4-Cyanophenyl]-3-(trifluoromethyl)-3H-diazirine; 4-(3-(Trifluoromethyl)diazirin-3-yl)benzonitrile; 3-(4-Cyanophenyl)-3-(trifluoromethyl)diaziridine; p-Cyanophenyltrifluoromethyldiazirine; TFD Benzonitrile; Diazirine-BCN; Photoaffinity Labeling Reagent 128886-91-9
EINECS	Contact for details

Quality Control

Our 4-(3-(Trifluoromethyl)-3H-Diazirin-3-Yl)Benzonitrile is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive research applications. We provide comprehensive analytical data, including HPLC purity, NMR, and MS confirmation. A Certificate of Analysis (COA) detailing all specifications is available with every shipment to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. This material is strictly light-sensitive and must be handled under appropriate conditions to maintain stability. Keep container tightly sealed in a dry and well-ventilated place.

Specification

Item	Specification
Appearance	White to off-white crystalline solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Melting Point	Contact for details
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.