Description

Phosphoprotein P65 CAS NO 128868-76-8 is a specific phosphoprotein of significant interest in biochemical and pharmaceutical research. This compound plays a crucial role in cellular signaling pathways, particularly those related to gene expression and immune responses. It is primarily utilized by researchers and manufacturers in the life sciences, biotechnology, and pharmaceutical development sectors for studying protein-protein interactions, kinase activity, and as a critical reagent in assay development.

Application

• Biochemical Research: Used as a key substrate or target protein in studies of phosphorylation dynamics, signal transduction, and NF-κB pathway analysis.
• Pharmaceutical Development: Serves as a critical reagent for high-throughput screening (HTS) assays to identify novel kinase inhibitors or modulators of inflammatory pathways.
• Diagnostic Reagent Production: Employed in the development and calibration of immunoassays (e.g., ELISA, Western Blot) for detecting specific phospho-epitopes.
• Cell Signaling Studies: Essential for in vitro and in vivo research models investigating apoptosis, cell proliferation, and immune cell activation.
• Proteomics & Biomarker Discovery: Used as a standard or bait protein in mass spectrometry-based proteomic studies to identify interacting partners and post-translational modifications.
• Antibody Validation: Acts as a positive control antigen for the validation and quality control of phospho-specific antibodies.

Basic Information

Product Name	Phosphoprotein P65
CAS No.	128868-76-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	p65 Phosphoprotein; NF-κB p65 (phosphorylated); RELA Phosphoprotein; Transcription factor p65 (phosphorylated); Nuclear factor NF-κ-B p65 subunit (phosphorylated); v-rel avian reticuloendotheliosis viral oncogene homolog A (phosphorylated); NFKB3 (phosphorylated); p65-NF-κB
EINECS	Contact for details

Quality Control

Our Phosphoprotein P65 is produced and handled under stringent quality management systems. Each batch undergoes rigorous analytical testing, including purity assessment by HPLC and identity confirmation via mass spectrometry and immunological methods, to ensure consistency and biological activity. Certificates of Analysis (COA) detailing specific purity, phosphorylation status, and functional activity data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake. Aliquot into single-use quantities to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or solution
Identification (MS)	Conforms
Identification (Immunoblot)	Reactive with anti-p65 and phospho-specific antibodies
Purity (SDS-PAGE)	≥ 90%
Purity (HPLC)	≥ 95%
Phosphorylation Status	Verified (Site-specific analysis available)
Biological Activity (Functional Assay)	Contact for details
Endotoxin Level	< 1.0 EU/μg (for cell culture grades)
Solubility	Specified buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.