Description

5-(And-6)-Carboxy-2,7-Dichlorofluorescein Diacetate is a chemically modified, cell-permeable derivative of fluorescein, designed for advanced research applications. This compound is critical for creating highly sensitive and specific fluorescent probes used in vital cellular assays. It is an essential reagent for researchers and manufacturers in the life sciences, pharmaceutical development, and diagnostic industries who require reliable, high-purity fluorescent indicators.

Application

• Fluorescent Probe Synthesis: A key precursor for preparing reactive derivatives like succinimidyl esters (SE) used to label proteins, antibodies, and other biomolecules.
• Intracellular Esterase Activity Assays: Used as a substrate to measure esterase activity within live cells; the non-fluorescent diacetate form is cleaved by intracellular esterases to yield the fluorescent carboxy-dichlorofluorescein.
• Reactive Oxygen Species (ROS) Detection: Serves as a foundational component in probes like H2DCFDA (2',7'-Dichlorodihydrofluorescein diacetate) for detecting and quantifying oxidative stress in cellular models.
• Flow Cytometry & Microscopy: Enables the development of fluorescent markers for cell sorting, viability assays, and high-resolution imaging studies.
• Diagnostic Kit Manufacturing: Incorporated into the production of research-use-only (RUO) and in-vitro diagnostic (IVD) kits for clinical and biomedical testing.
• Biochemical Research: Employed in enzyme kinetics studies and the development of novel fluorescence-based detection methodologies.

Basic Information

Product Name	5-(And-6)-Carboxy-2,7-Dichlorofluorescein Diacetate
CAS No.	127770-45-0
Molecular Formula	C25H16Cl2O9
Molecular Weight	531.30 g/mol
Synonyms	5(6)-Carboxy-2',7'-Dichlorofluorescein Diacetate; 5/6-Carboxy-DCFDA; CADCFDA; 2',7'-Dichloro-5(6)-carboxyfluorescein Diacetate; 5-(and-6)-Carboxy-2',7'-dichloro-3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl diacetate; DCFDA Carboxy Derivative; 5,6-Carboxy-DCFDA; Dichlorodihydrofluorescein Diacetate Carboxylic Acid
EINECS	Contact for details

Quality Control

Our 5-(And-6)-Carboxy-2,7-Dichlorofluorescein Diacetate is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity for sensitive research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is light-sensitive (store away from light). For long-term stability, consider storage under inert atmosphere and desiccation to maintain optimal performance.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Solubility	Soluble in DMSO, DMF; sparingly soluble in ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.