Description

2,3-Bis(3-Methylphenyl)-5-Phenyltetrazolium Chloride is a specialized tetrazolium salt widely utilized as a vital reagent in biochemical and analytical applications. Its core value lies in its role as a redox indicator and chromogenic substrate, enabling the sensitive detection and quantification of biological activity. This compound is essential for researchers and quality control professionals in the pharmaceutical, biotechnology, and diagnostic industries, where precise measurement of cellular viability and enzymatic function is critical.

Application

• Cell Viability and Cytotoxicity Assays: Used as a key substrate in MTT and related assays to measure mitochondrial activity in live cells.
• Biochemical Research: Serves as an electron acceptor in dehydrogenase enzyme assays for studying metabolic pathways.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to evaluate the efficacy and toxicity of drug candidates.
• Microbiology: Applied in microbial viability tests and for assessing biofilm metabolic activity.
• Diagnostic Kit Manufacturing: Incorporated as a critical component in diagnostic kits for clinical and research use.
• Material Science: Potential use in developing sensors and indicators due to its colorimetric properties upon reduction.

Basic Information

Product Name	2,3-Bis(3-Methylphenyl)-5-Phenyltetrazolium Chloride
CAS No.	127697-61-4
Molecular Formula	C27H22ClN4
Molecular Weight	437.94 g/mol
Synonyms	2,3-Bis(3-methylphenyl)-5-phenyl-2H-tetrazolium chloride; 3-(3-Methylphenyl)-2,5-diphenyl-2H-tetrazolium chloride; m-Tetranitro Blue Tetrazolium Chloride (related analog); TNBT Chloride analog; Tetrazolium Blue derivative; Redox Indicator Salt; Viability Assay Reagent; Biochemical Chromogen
EINECS	Contact for details

Quality Control

Every batch of 2,3-Bis(3-Methylphenyl)-5-Phenyltetrazolium Chloride is manufactured under strict quality management systems. We ensure the product meets high-purity standards suitable for sensitive research and diagnostic applications. Comprehensive analytical testing, including HPLC for purity and identity confirmation, is performed. A Certificate of Analysis (COA) detailing batch-specific results is provided with each shipment to guarantee traceability and compliance with your specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to minimize exposure to atmospheric humidity. For long-term stability, consider storage under an inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.1%
Solubility	Soluble in DMSO, methanol; slightly soluble in water
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.