Description

5α,6Beta-Dihydroxycholestanol-D7 is a high-purity, deuterium-labeled sterol derivative, specifically designed for use as an internal standard in advanced analytical chemistry. This compound is critical for ensuring the accuracy and reliability of quantitative analyses in research and quality control laboratories. It is primarily utilized by scientists and manufacturers in the pharmaceutical, biotechnology, and clinical diagnostics sectors for precise measurement and method validation.

Application

• Internal Standard for LC-MS/MS: Essential for the accurate quantification of cholesterol and its metabolites in complex biological matrices like plasma, serum, and tissues.
• Metabolomics Research: Used as a stable isotope-labeled tracer to study cholesterol biosynthesis pathways and related metabolic disorders.
• Pharmaceutical Development: Supports method development and validation in drug discovery programs targeting lipid metabolism and cardiovascular diseases.
• Clinical Diagnostics: Enables precise calibration in assays for diagnosing inborn errors of metabolism, such as Smith-Lemli-Opitz syndrome.
• Biochemical Research: Serves as a key reference material in enzymatic studies and the investigation of sterol-protein interactions.
• Quality Control & Assurance: Provides a definitive standard for ensuring batch-to-batch consistency in the production of related sterol-based compounds.

Basic Information

Product Name	5α,6Beta-Dihydroxycholestanol-D7
CAS No.	127684-07-5
Molecular Formula	C₂₇H₄₁D₇O₃
Molecular Weight	~419.7 g/mol
Synonyms	5α,6β-Dihydroxycholestan-3β-ol-D7; (3β,5α,6β)-Cholestane-3,5,6-triol-D7; Deuterated 5,6-Dihydroxycholestanol; Cholestane-3β,5α,6β-triol-d7; 5α,6β-Dihydroxycholestanol (D7); [D7]-5α,6β-Dihydroxycholestanol; Internal Standard for Cholesterol Metabolite Analysis
EINECS	Contact for details

Quality Control

Every batch of 5α,6Beta-Dihydroxycholestanol-D7 is manufactured and tested under strict quality management systems. We provide comprehensive analytical data to ensure the product meets the high standards required for research and diagnostic applications. A detailed Certificate of Analysis (COA), including results for isotopic purity, chemical purity, and identity confirmation, is supplied with each shipment and is available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-content)	≥ 98 atom % D
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.