Description

Dabcyl-γ-Abu-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Edans is a synthetic, fluorogenic peptide substrate specifically engineered for protease activity assays. This compound matters because it enables highly sensitive, real-time detection and quantification of enzymatic activity, particularly for HIV-1 protease and related enzymes, through Förster Resonance Energy Transfer (FRET). Pharmaceutical researchers, diagnostic developers, and academic biochemists need it for critical applications in drug discovery, mechanistic studies, and high-throughput screening.

Application

• HIV-1 Protease Assays: Primary substrate for measuring the catalytic activity and inhibition of HIV-1 protease in drug discovery and biochemical research.
• FRET-Based Protease Screening: Enables high-throughput screening (HTS) of compound libraries for protease inhibitors using fluorescence signal generation upon cleavage.
• Enzyme Kinetics Studies: Used to determine kinetic parameters (Km, Vmax, kcat) and study the mechanism of action of viral and other proteases.
• Diagnostic Development: Serves as a core component in developing fluorescence-based diagnostic tests for protease-related biomarkers.
• Biochemical Research: A vital tool for studying protease specificity, function, and regulation in various biological pathways.
• Quality Control in Peptide Synthesis: Can be used as a reference standard or model substrate to validate the performance of synthetic and analytical processes.

Basic Information

Product Name	Dabcyl-γ-Abu-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Edans
CAS No.	127134-13-8
Molecular Formula	C73H102N18O23S
Molecular Weight	1583.8 g/mol
Synonyms	Dabcyl-γ-Abu-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Edans; HIV-1 Protease Substrate (Dabcyl-Edans); FRET Substrate for HIV-1 Protease; 5-((2-Aminoethyl)amino)naphthalene-1-sulfonyl-Gln-Asn-Tyr-Pro-Ile-Val-Gln-4-((4-(dimethylamino)phenyl)azo)benzoic Acid; EDANS-Dabcyl FRET Peptide; γ-Aminobutyric acid-containing HIV Protease Substrate; Mca-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 (related class).
EINECS	Contact for details

Quality Control

Our Dabcyl-γ-Abu-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Edans is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and mass spectrometry for identity confirmation, to ensure it meets the high standards required for sensitive biochemical assays. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Off-white to light yellow solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular weight
Purity (HPLC)	≥ 95% (Area percent at 220 nm & 280 nm)
Water Content (KF)	≤ 5.0 %
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Cleavable by HIV-1 protease with characteristic fluorescence increase

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.