Description

Endoflagellar Sheath Protein, Treponema Pallidum is a highly purified, recombinant protein derived from the causative agent of syphilis. This specialized biochemical is a critical research tool for the study of bacterial motility, pathogenesis, and host immune response mechanisms. It is primarily utilized by research institutions, diagnostic developers, and pharmaceutical companies engaged in infectious disease research and vaccine development.

Application

• Antigen for Immunoassays: Used as a key coating antigen in enzyme-linked immunosorbent assays (ELISA) and other serological tests for the detection of Treponema pallidum-specific antibodies.
• Pathogenesis Research: Essential for studying the role of the endoflagellar sheath in bacterial motility, colonization, and immune evasion strategies of T. pallidum.
• Vaccine Development: Serves as a candidate antigen in preclinical research for the development of novel syphilis vaccines, targeting the bacterium's structural components.
• Biomarker Discovery: Employed in proteomic and immunological studies to identify and characterize novel diagnostic biomarkers for syphilis and related treponemal infections.
• Antibody Production: Used to immunize animals for the generation of polyclonal or monoclonal antibodies specific to T. pallidum sheath proteins.
• Structural Biology Studies: Provides material for X-ray crystallography, cryo-electron microscopy, and other techniques to elucidate the protein's three-dimensional structure and function.

Basic Information

Product Name	Endoflagellar Sheath Protein, Treponema Pallidum
CAS No.	126468-97-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Treponema pallidum Endoflagellar Sheath Protein; Tp Endoflagellar Sheath Antigen; Tp Sheath Protein; Flagellar Sheath Protein (Treponema pallidum); Recombinant Tp Sheath Protein; Syphilis Research Antigen; Tp FlaA3 (potential related protein); Periplasmic Flagellar Sheath Protein
EINECS	Contact for details

Quality Control

Our Endoflagellar Sheath Protein is produced under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot is subjected to rigorous purity analysis, including SDS-PAGE and HPLC, and tested for endotoxin levels to ensure suitability for immunological applications. A comprehensive Certificate of Analysis (COA), detailing purity, identity (by Western Blot or Mass Spectrometry), and functional activity data, is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Reconstituted protein aliquots should be stored at -70°C to -80°C and avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the vial is equilibrated to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Western Blot)	Reacts with specific anti-T. pallidum antisera
Purity (SDS-PAGE)	≥ 90%
Protein Content (BCA)	≥ 95% of total weight
Endotoxin (LAL)	< 1.0 EU/µg
Solubility	Soluble in PBS or appropriate aqueous buffer
Biological Activity (ELISA)	Binds specifically to syphilis-positive human sera

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.