Description

Human Mcp-1 is a crucial chemokine protein, also known as CCL2, that plays a central role in the immune system's inflammatory response by recruiting monocytes, memory T cells, and dendritic cells to sites of tissue injury or infection. Its precise biological activity makes it an indispensable reagent for life science research, drug discovery, and diagnostic development. This high-purity protein is essential for researchers and manufacturers in the fields of immunology, oncology, and autoimmune disease research, where understanding and modulating inflammatory pathways is critical.

Application

• Biomedical Research: Used as a key cytokine in in vitro cell culture studies to investigate monocyte and macrophage migration, activation, and signaling pathways.
• Drug Discovery & Screening: Serves as a target or tool compound in high-throughput screening assays to identify and validate potential anti-inflammatory therapeutics.
• Diagnostic Development: Employed as a critical component in the calibration and validation of immunoassays (e.g., ELISA) designed to measure MCP-1 levels in clinical samples.
• Cell-Based Assays: Essential for functional assays in chemotaxis chambers (e.g., Boyden chamber) to study leukocyte migration and chemotaxis.
• Inflammation Modeling: Used to establish in vitro models of chronic inflammatory diseases, such as atherosclerosis, rheumatoid arthritis, and multiple sclerosis.
• Antibody Production & Validation: Acts as an immunogen for generating specific antibodies or as a standard for characterizing antibody specificity and affinity.

Basic Information

Product Name	Human Mcp-1
CAS No.	126463-99-8
Molecular Formula	C₆₇₀H₁₀₄₄N₁₈₄O₂₀₀S₁₀
Molecular Weight	Approx. 15 kDa (monomer)
Synonyms	Monocyte Chemoattractant Protein-1; CCL2; Small Inducible Cytokine A2; HC11; GDCF-2; MCAF; SMC-CF; JE Homolog; SCYA2; MCP1
EINECS	Contact for details

Quality Control

Our Human Mcp-1 is produced under stringent conditions to ensure the highest levels of purity and biological activity, suitable for sensitive research applications. Each batch is subjected to rigorous analytical testing, including SDS-PAGE, HPLC, and endotoxin level analysis, to guarantee consistency and meet exacting research standards. A comprehensive Certificate of Analysis (COA) detailing purity, concentration, biological activity (typically verified by cell migration assay), and sterility is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Upon reconstitution, aliquot to avoid repeated freeze-thaw cycles and store at -70°C to -80°C for optimal preservation of activity. This product is hygroscopic (moisture-sensitive).

Specification

Item	Specification
Appearance	White or off-white lyophilized powder
Purity (SDS-PAGE)	> 95%
Purity (HPLC)	> 98%
Molecular Weight	Approx. 15 kDa (reduced)
Endotoxin Level	< 1.0 EU/μg
Biological Activity	Measured by chemotaxis assay using THP-1 cells
Solubility	Soluble in water or PBS
Sterility (Bacterial/Fungal)	Sterile filtered, 0.2 μm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.