Description

H-Ala-Pro-Afc CAS NO 125791-92-6 is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control labs requiring precise detection and quantification of specific protease activity. It is primarily utilized in pharmaceutical research, biochemical studies, and diagnostic development for conditions involving proteolytic enzymes.

Application

• Protease Activity Assays: Used as a selective fluorogenic substrate for measuring the activity of dipeptidyl peptidases (DPP) and related proteases in research settings.
• Drug Discovery & Development: Employed in high-throughput screening (HTS) campaigns to identify and characterize potential enzyme inhibitors for therapeutic targets.
• Biochemical Research: Serves as a key tool for kinetic studies, enzyme characterization, and mechanistic investigations of protease function and regulation.
• Diagnostic Reagent Manufacturing: Incorporated into the development of diagnostic kits and reagents for clinical testing of enzymatic biomarkers.
• Cell Biology Studies: Applied in live-cell imaging and flow cytometry to monitor protease activity within cellular pathways and processes.
• Quality Control (QC) Testing: Used in QC laboratories for the potency testing of biopharmaceuticals where specific protease activity is a critical quality attribute.

Basic Information

Product Name	H-Ala-Pro-Afc
CAS No.	125791-92-6
Molecular Formula	C₁₉H₂₄N₄O₅
Molecular Weight	388.42 g/mol
Synonyms	Ala-Pro-AFC; H-Ala-Pro-7-Amino-4-trifluoromethylcoumarin; L-Alanyl-L-prolyl-7-amino-4-trifluoromethylcoumarin; Ala-Pro-AFC trifluoroacetate salt; DPP-IV Substrate (AFC); Dipeptidyl Peptidase IV Fluorogenic Substrate; 7-Amino-4-trifluoromethylcoumarin-Ala-Pro; AFC-based DPP substrate
EINECS	Contact for details

Quality Control

Our H-Ala-Pro-Afc is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. Due to its hygroscopic (moisture-sensitive) nature, allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.