Description

D1 Photosystem II Protein, Plant is a highly purified, functional protein complex isolated from the photosynthetic apparatus of plants. This critical component is essential for fundamental research into the light reactions of photosynthesis, specifically water oxidation and oxygen evolution. It is a vital reagent for biochemists, molecular biologists, and plant physiologists studying electron transport mechanisms, herbicide action, and environmental stress responses in photosynthetic organisms.

Application

• Biochemical Research: Fundamental studies on the structure, function, and assembly of Photosystem II (PSII).
• Mechanistic Studies: Investigation of the water-splitting (oxygen-evolving) complex and electron transfer pathways.
• Herbicide Mode-of-Action Research: Target-site studies for herbicides that inhibit the QB-binding site of the D1 protein (e.g., triazines, ureas).
• Environmental Stress Analysis: Research on how abiotic stresses (e.g., high light, temperature, UV) affect PSII integrity and D1 protein turnover.
• Enzyme Kinetics: Assays to measure photosynthetic electron transport rates and inhibitor constants (Ki).
• Biomimetic System Development: As a component in synthetic systems designed to mimic natural photosynthesis for energy applications.
• Educational Purposes: High-grade material for advanced university laboratory courses in plant biochemistry and biophysics.

Basic Information

Product Name	D1 Photosystem II Protein, Plant
CAS No.	125389-73-3
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	PSII D1 Protein; Photosystem II D1 Reaction Center Protein; D1 Polypeptide of Photosystem II; Herbicide-Binding Protein; psbA Gene Product; QB-Protein; 32 kDa Herbicide-Binding Protein; Chloroplast-Encoded D1 Protein
EINECS	Contact for details

Quality Control

Our D1 Photosystem II Protein is produced and handled under stringent conditions to ensure biological activity and purity. Each batch is characterized for functional integrity, typically via oxygen evolution assays or electron transport measurements. Purity is assessed by SDS-PAGE and immunoblotting. A comprehensive Certificate of Analysis (COA) detailing specific activity, purity, buffer composition, and storage conditions is provided with every shipment to support your research reproducibility.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C to -80°C in small aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive). Thaw on ice immediately before use. Do not expose to elevated temperatures.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Identification (SDS-PAGE/Immunoblot)	Corresponds to D1 protein (~32 kDa)
Purity (by SDS-PAGE)	≥ 90% (D1 band)
Specific Activity (O2 Evolution)	Contact for details (μmol O2/mg protein/hr)
Buffer	Specified storage buffer (e.g., HEPES, Sucrose, MgCl2)
Protein Concentration	Contact for details (mg/mL)
Preservative	None
Biological Source	Spinach (Spinacia oleracea) or as specified

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.