Description

Vinculin is a high-purity biochemical reagent and research chemical, identified by CAS No. 125361-02-6. This compound is a critical tool for researchers studying cell adhesion, cytoskeletal dynamics, and mechanotransduction pathways. It is essential for life science research, pharmaceutical development, and diagnostic applications where understanding cell-matrix interactions is paramount.

Application

• Biomedical Research: Used as a key antigen for the study of focal adhesions, cell signaling, and the role of the cytoskeleton in cellular structure and motility.
• Antibody Production & Validation: Serves as a standard or immunogen for the development and quality control of monoclonal and polyclonal antibodies targeting vinculin.
• Diagnostic Assay Development: Employed in the research and calibration of ELISA, Western blot, and immunohistochemistry (IHC) assays for detecting vinculin expression in biological samples.
• Pharmaceutical Screening: Utilized in target-based screening platforms to identify potential therapeutic compounds that modulate cell adhesion processes.
• Cell Biology Studies: Fundamental for experiments investigating the effects of genetic manipulation, drug treatment, or disease states on focal adhesion plaque composition and function.
• Academic & Institutional Research: A staple reagent in university and government laboratories focused on cancer research, cardiovascular biology, and developmental biology.

Basic Information

Product Name	Vinculin
CAS No.	125361-02-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Metavinculin; VCL; Vinculin (Metavinculin); Cell adhesion protein vinculin; 125361-02-6; Focal adhesion protein vinculin
EINECS	Contact for details

Quality Control

Our Vinculin is produced and handled under strict quality management protocols to ensure batch-to-batch consistency and reliability for critical research applications. Each lot undergoes comprehensive analytical testing, including purity assessment and functional activity checks where applicable. A detailed Certificate of Analysis (COA) documenting identity, purity, and other relevant parameters is provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For optimal performance, prepare aliquots to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or solution
Identification (SDS-PAGE)	Complies
Purity (SDS-PAGE)	≥ 90%
Protein Concentration	As per COA
Buffer Composition	Specified on COA
Biological Activity	Functional in standard assay (e.g., binding assay)
Endotoxin Level	< 1.0 EU/µg (if applicable)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.