Description

4-Methylumbelliferyl Phosphate, Dilithium Salt is a high-purity, water-soluble fluorogenic substrate essential for enzymatic assays. Its core value lies in its ability to generate a highly fluorescent product upon dephosphorylation, enabling sensitive and quantitative detection of enzyme activity. This compound is critically needed by researchers and quality control laboratories in the pharmaceutical, diagnostic, and life science research sectors for applications such as phosphatase activity measurement and high-throughput screening.

Application

• Key Substrate for Phosphatase Assays: Primarily used as a fluorogenic substrate for detecting and quantifying the activity of alkaline phosphatase (ALP), acid phosphatase, and other phosphatases in research and diagnostic kits.
• Diagnostic Reagent Manufacturing: A critical raw material in the production of clinical chemistry reagents for automated analyzers to measure enzyme levels in serum, a vital diagnostic marker.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to identify and characterize inhibitors or activators of phosphatase enzymes, which are important therapeutic targets.
• Cell Biology & Signal Transduction Research: Used to study cellular processes involving phosphorylation/dephosphorylation cycles within live cells or cell lysates.
• Quality Control in Biotechnology: Applied in the QC testing of enzyme preparations, biologics, and other products where phosphatase activity must be monitored or eliminated.
• Environmental & Food Safety Testing: Utilized in enzymatic methods developed to detect certain contaminants or biomarkers in environmental samples and food products.

Basic Information

Product Name	4-Methylumbelliferyl Phosphate, Dilithium Salt
CAS No.	125328-83-8
Molecular Formula	C10H7Li2O7P
Molecular Weight	284.02 g/mol
Synonyms	4-MUP, Dilithium Salt; 4-Methylumbelliferyl Phosphate Disodium Salt (common misnomer); 4-Methylumbelliferyl Dihydrogen Phosphate, Dilithium Salt; 7-Hydroxy-4-methylcoumarin Phosphate, Dilithium Salt; 4-MU-PO4; 4-Methylumbelliferyl Phosphate; Phosphoric Acid Mono-(4-methyl-2-oxo-2H-chromen-7-yl) Ester, Dilithium Salt; Fluorophore-Phosphate Conjugate
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl Phosphate, Dilithium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive enzymatic applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to meet exacting research and diagnostic standards. Certificates of Analysis (COA) with detailed specifications are provided to guarantee compliance with your application requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Fluorogenic Activity	Conforms to reference standard
Solubility (in Water)	Clear, colorless solution at 10 mg/mL

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.