Description

Prp20 Protein CAS NO 125268-44-2 is a highly purified recombinant protein, specifically the yeast homolog of the mammalian RNA polymerase II-associated protein 20 (RPAP2). This protein plays a critical role in the assembly and regulation of the RNA polymerase II complex, making it an essential reagent for fundamental research in transcription and gene expression. It is primarily utilized by researchers in molecular biology, biochemistry, and pharmaceutical development who require high-purity, functionally active proteins for their studies. Its consistent quality and biological activity are vital for ensuring reproducible experimental results in both academic and industrial R&D settings.

Application

• Biochemical Research: Used as a key component in in vitro transcription assays to study the mechanisms of RNA polymerase II assembly and function.
• Protein-Protein Interaction Studies: Serves as a bait or prey protein in pull-down, co-immunoprecipitation (Co-IP), or yeast two-hybrid screens to map the interactome of the transcription machinery.
• Enzymology: Employed in enzymatic studies to investigate the kinetics and regulation of the RNA polymerase II holoenzyme complex formation.
• Antigen Production: Can be used to generate specific polyclonal or monoclonal antibodies for the detection and quantification of RPAP2 in various biological samples.
• Cell Biology: Applied in functional complementation assays in yeast or other model systems to elucidate the in vivo role of Prp20/RPAP2.
• Drug Discovery Screening: Potential use as a target protein in high-throughput screening assays to identify novel compounds that modulate transcription.

Basic Information

Product Name	Prp20 Protein
CAS No.	125268-44-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Recombinant Prp20 Protein; Yeast RNA Polymerase II-Associated Protein 20; Rtr1; RPAP2 (Yeast homolog); RNA polymerase II-associated protein RTR1; YDR404C; SGD:S000002735; Transcription factor Rtr1
EINECS	Contact for details

Quality Control

Our Prp20 Protein is manufactured under stringent conditions to ensure the highest standards of purity and biological activity. Each batch undergoes rigorous quality testing, including analysis for identity, purity, and functional activity. Certificates of Analysis (COA) detailing specifications such as purity by SDS-PAGE, endotoxin levels, and activity in functional assays are available upon request. We are committed to providing research-grade materials that meet the exacting requirements of the scientific community.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation.

Specification

Item	Specification
Appearance	White or off-white lyophilized powder or clear solution
Identification (SDS-PAGE)	Single band at expected molecular weight
Purity (SDS-PAGE)	> 95%
Protein Content (Bradford/UV)	As per COA
Endotoxin Level (LAL)	< 1.0 EU/µg
Biological Activity	Functional in in vitro transcription or binding assay
Solubility	Soluble in appropriate assay buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.