Description

3'-Hydroxywarfarin is a key hydroxylated metabolite of the anticoagulant drug warfarin, identified by CAS No. 124952-36-9. This compound is of significant interest in pharmaceutical research and development for studying drug metabolism, pharmacokinetics, and pharmacogenetics. It serves as a critical reference standard and intermediate for analytical laboratories, toxicology studies, and manufacturers of active pharmaceutical ingredients (APIs).

Application

• Pharmaceutical Reference Standard: Primary use as a certified reference material (CRM) for the quantitative and qualitative analysis of warfarin and its metabolites in biological matrices.
• Drug Metabolism & Pharmacokinetics (DMPK) Studies: Essential for in vitro and in vivo studies to understand the metabolic pathways, clearance, and bioavailability of warfarin.
• Analytical Method Development: Used to develop and validate sensitive HPLC, LC-MS/MS, and GC-MS methods for clinical and forensic toxicology.
• Pharmacogenetics Research: Investigates the impact of genetic polymorphisms (e.g., in CYP2C9 enzyme) on warfarin metabolism and dosing requirements.
• Impurity Profiling: Serves as a specified impurity standard in the quality control of warfarin API manufacturing to ensure product purity and safety.
• Biochemical Research: Used as a tool compound in enzymology studies related to vitamin K epoxide reductase and other relevant biological targets.

Basic Information

Product Name	3'-Hydroxywarfarin
CAS No.	124952-36-9
Molecular Formula	C19H16O6
Molecular Weight	340.33 g/mol
Synonyms	3'-Hydroxywarfarin; 4-Hydroxy-3-(3-hydroxy-1-phenylbutyl)-2H-1-benzopyran-2-one; Warfarin, 3'-hydroxy-; 3-Hydroxywarfarin (positional isomer); 3'-OH-Warfarin; Warfarin 3'-Alcohol; 3'-Hydroxy-4-hydroxy-3-(3-oxo-1-phenylbutyl)coumarin (related form).
EINECS	Contact for details

Quality Control

Our 3'-Hydroxywarfarin is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by spectroscopic methods (IR, NMR, MS), to ensure it meets the high standards required for research and reference standard applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profile is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept sealed in a desiccated environment away from direct light exposure to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0% Any single unknown impurity ≤ 0.5%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.