Description

Hmga1A Protein CAS NO 124544-67-8 is a high-purity, recombinant protein corresponding to the High Mobility Group AT-hook 1 isoform A, a key architectural transcription factor involved in chromatin remodeling and gene regulation. This product is essential for researchers and developers requiring a reliable and well-characterized reagent for studying gene expression, cellular differentiation, and oncogenic processes. It is primarily utilized in life science research, pharmaceutical development for target validation, and diagnostic assay development.

Application

• Biochemical Research: Used as a critical reagent in chromatin immunoprecipitation (ChIP), electrophoretic mobility shift assays (EMSA), and protein-protein interaction studies to investigate DNA binding and transcriptional regulation.
• Drug Discovery & Target Validation: Serves as a target protein in high-throughput screening (HTS) assays to identify potential therapeutic compounds that modulate its activity in cancer and other diseases.
• Diagnostic Development: Employed as a standard or coating antigen in the development of immunoassays (ELISA) for detecting autoantibodies or quantifying HMGA1 levels in clinical samples.
• Cell Biology Studies: Utilized in cellular transfection and functional studies to elucidate the role of HMGA1 in processes like epithelial-mesenchymal transition (EMT), cell proliferation, and apoptosis.
• Protein Interaction Mapping: Acts as bait or prey in pull-down assays and mass spectrometry-based proteomics to map its interactome and understand signaling networks.
• Antibody Production & Validation: Functions as a high-quality immunogen for generating specific polyclonal or monoclonal antibodies, and as a positive control for antibody validation.

Basic Information

Product Name	Hmga1A Protein
CAS No.	124544-67-8
Molecular Formula	Contact for details
Molecular Weight	~11.6 kDa (calculated for human HMGA1a, 107 aa)
Synonyms	High Mobility Group Protein HMG-I/HMG-Y
HMGA1 Protein
High Mobility Group AT-Hook 1 Isoform A
HMG-I(Y) Protein
Recombinant Human HMGA1a Protein
AT-Hook Transcription Factor
HMG-IC (historical)
EINECS	Contact for details

Quality Control

Our Hmga1A Protein is produced under stringent quality systems to ensure batch-to-batch consistency and high biological activity. Each lot is subjected to rigorous purity and identity testing, including SDS-PAGE, Western Blot, and mass spectrometry. Functional activity is confirmed via DNA-binding assays. A comprehensive Certificate of Analysis (COA) detailing purity, concentration, endotoxin levels, and buffer composition is provided with every shipment, supporting research reproducibility and compliance.

Storage

Preserve in a tightly closed container, protected from light. For long-term storage, aliquot and store at ≤ -20°C or -80°C. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the vial is equilibrated to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Clear, colorless solution
Purity (SDS-PAGE)	≥ 95%
Identification (Western Blot)	Positive
Protein Concentration	≥ 0.5 mg/mL
Endotoxin Level	< 1.0 EU/μg
Buffer	Specified phosphate or Tris-based buffer
Biological Activity (EMSA)	Confirmed

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.