Description

Coelenterazine Hcp is a high-purity, synthetic luciferin analog widely used as a critical substrate in bioluminescence assays. Its primary value lies in its high quantum yield and stability, enabling highly sensitive detection of calcium ions and reactive oxygen species in cellular environments. This compound is essential for researchers and manufacturers in the fields of molecular biology, drug discovery, and diagnostic assay development, where reliable and reproducible luminescent signals are paramount.

Application

• Bioluminescence Resonance Energy Transfer (BRET) Assays: A key substrate for studying protein-protein interactions and intracellular signaling pathways in live cells.
• Calcium Ion Sensing: Used with aequorin and other photoproteins as a highly sensitive intracellular calcium indicator.
• Reactive Oxygen Species (ROS) Detection: Employed in assays to measure oxidative stress and antioxidant activity in biological samples.
• High-Throughput Screening (HTS): Integral to luciferase-based reporter assays for drug discovery and genetic research.
• In Vivo Imaging: Serves as a substrate for engineered luciferases in small animal imaging to monitor tumor growth, gene expression, and infection progression.
• Diagnostic Kit Manufacturing: A critical raw material in the production of commercial diagnostic and research kits for cellular analysis.

Basic Information

Product Name	Coelenterazine Hcp
CAS No.	123437-32-1
Molecular Formula	C26H21N3O2
Molecular Weight	407.47 g/mol
Synonyms	Coelenterazine-hcp; DeepBlueC™; 2-Deoxycoelenterazine; 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one; 8-Benzyl-6-(4-hydroxyphenyl)-2-[(4-hydroxyphenyl)methyl]imidazo[1,2-a]pyrazin-3(7H)-one; CLZN-hcp; h-CTZ
EINECS	Contact for details

Quality Control

Every batch of Coelenterazine Hcp is manufactured under strict quality management systems and undergoes comprehensive analytical testing. We guarantee high chemical purity and performance consistency suitable for sensitive research and in vitro diagnostic (IVD) applications. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, purity (by HPLC), and absence of critical impurities.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. Due to its hygroscopic and strictly light-sensitive nature, the product should be handled in a controlled environment with minimal exposure to ambient light and moisture. Allow the vial to equilibrate to room temperature in a dark place before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Bioluminescence Activity	Conforms to reference (by customer assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.