Description

2,3-Bis(4-Chlorophenyl)-5-Cyano-2H-Tetrazolium Chloride is a specialized tetrazolium salt derivative, primarily valued as a critical reagent in biochemical and analytical chemistry. Its core value proposition lies in its role as a redox indicator and chromogenic substrate, enabling sensitive detection and quantification in enzymatic assays. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic industries, where precise measurement of cellular activity and enzyme function is required.

Application

• Biochemical Assay Reagent: Used as a vital chromogenic substrate in cell viability assays, such as MTT assay alternatives, for measuring mitochondrial activity.
• Enzymatic Analysis: Serves as an electron acceptor in dehydrogenase enzyme assays, facilitating colorimetric detection for research and diagnostic purposes.
• Pharmaceutical Research: Employed in high-throughput screening (HTS) platforms to evaluate the cytotoxic effects of new drug candidates.
• Microbiology: Applied in microbial viability testing and for studying metabolic activity in bacterial and fungal cultures.
• Diagnostic Kits: Incorporated as a key component in commercial diagnostic test kits for clinical and research laboratories.
• Material Science Research: Utilized in studies involving redox-active materials and polymer chemistry.

Basic Information

Product Name	2,3-Bis(4-Chlorophenyl)-5-Cyano-2H-Tetrazolium Chloride
CAS No.	123431-15-2
Molecular Formula	C21H12Cl3N5
Molecular Weight	432.71 g/mol
Synonyms	2,3-Bis(p-chlorophenyl)-5-cyanotetrazolium Chloride; 2,3-Bis(4-Chlorophenyl)-5-cyanotetrazolium Chloride; 5-Cyano-2,3-bis(4-chlorophenyl)-2H-tetrazolium Chloride; Tetrazolium Chloride, 2,3-bis(4-chlorophenyl)-5-cyano-; CTC (context-specific abbreviation); NSC 401042; 2,3-Di(4-chlorophenyl)-5-cyanotetrazolium Chloride
EINECS	Contact for details

Quality Control

Our production of 2,3-Bis(4-Chlorophenyl)-5-Cyano-2H-Tetrazolium Chloride adheres to stringent quality protocols to ensure batch-to-batch consistency and high purity for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are provided to guarantee the material meets the exacting standards required for biochemical and pharmaceutical research.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term storage, consider desiccants or inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.