Description

Hisactophilin is a small, pH-sensitive actin-binding protein originally isolated from the slime mold *Dictyostelium discoideum*. Its unique ability to bind and bundle actin filaments in a pH-dependent manner makes it a valuable biochemical tool for studying cytoskeletal dynamics and membrane-cytoskeleton interactions. This compound is primarily utilized by researchers in cell biology, biochemistry, and biophysics for in vitro assays and mechanistic studies.

Application

• Biochemical Research: Used as a model protein to study the mechanisms of pH-sensitive protein-lipid and protein-cytoskeleton interactions.
• In Vitro Cytoskeleton Studies: Employed in actin polymerization and depolymerization assays to investigate the regulation of the actin cytoskeleton.
• Membrane Biophysics: Serves as a tool to probe the coupling between lipid membranes and the actin network under varying pH conditions.
• Protein Engineering & Design: Acts as a scaffold or module in the design of novel pH-responsive biomaterials or biosensors.
• Academic & Pharmaceutical R&D: Facilitates fundamental research into cellular motility, shape changes, and intracellular trafficking processes relevant to disease mechanisms.

Basic Information

Product Name	Hisactophilin
CAS No.	122962-20-3
Molecular Formula	Contact for details
Molecular Weight	~13.9 kDa (Protein)
Synonyms	Hisactophilin I; pH-sensitive actin-binding protein; Histidine-rich actin-binding protein; DdHisactophilin; Dictyostelium hisactophilin
EINECS	Contact for details

Quality Control

Our Hisactophilin is produced and handled under controlled conditions to ensure batch-to-batch consistency and biological activity. Each lot undergoes rigorous quality testing, including assessment of purity, identity, and functional activity in standard actin-binding assays. Certificates of Analysis (COA) detailing specific test results are available upon request to support your research and documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to reach room temperature in a desiccator before opening to prevent condensation and moisture uptake. For extended storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/MS)	Conforms to standard
Purity (SDS-PAGE/HPLC)	≥ 90%
Protein Content (Bradford/UV)	≥ 95% of dry weight
Biological Activity	pH-dependent actin bundling activity confirmed
Endotoxin Level	< 1.0 EU/mg
Solubility	Soluble in appropriate aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.