Description

Antithrombin Chicago is a specific variant of the serine protease inhibitor antithrombin, a key regulator of the blood coagulation cascade. This high-purity biological compound is critical for research and development in hematology and thrombosis, providing a precise tool for studying coagulation disorders and inhibitor mechanisms. It is essential for pharmaceutical R&D teams, diagnostic kit manufacturers, and academic researchers focused on cardiovascular disease, hemostasis, and novel anticoagulant therapies.

Application

• Biochemical Research: Used as a critical reference standard and reagent in studies of blood coagulation pathways, serine protease inhibition kinetics, and structure-function relationships of antithrombin variants.
• Pharmaceutical Development: Serves as a key material in the research and preclinical testing of new anticoagulant and antithrombotic drugs, including heparin potentiators.
• Diagnostic Manufacturing: Employed as a calibrated component in the production of in-vitro diagnostic (IVD) assays and kits for measuring antithrombin activity or antigen levels in human plasma.
• Disease Mechanism Studies: Facilitates investigation into hereditary thrombophilia linked to antithrombin deficiency or dysfunction, including the specific "Chicago" mutation.
• Quality Control & Standardization: Acts as a purity standard for lot-to-lot consistency testing of therapeutic antithrombin concentrates and related biological products.

Basic Information

Product Name	Antithrombin Chicago
CAS No.	122653-64-9
Molecular Formula	Contact for details
Molecular Weight	~58 kDa (Approx., Glycoprotein)
Synonyms	Antithrombin III Chicago Variant; ATIII Chicago; Serpin C1 Chicago Mutant; Antithrombin (AT) Chicago; AT Chicago; Antithrombin III (p.Leu131Phe); AT III Variant Chicago; Antithrombin, Chicago Type
EINECS	Contact for details

Quality Control

Our Antithrombin Chicago is produced and tested under stringent conditions to ensure the highest standards of purity and biological activity. Each batch is characterized using advanced analytical techniques, including SDS-PAGE, HPLC, and functional activity assays. A comprehensive Certificate of Analysis (COA) detailing purity, potency, and identity is provided with every shipment to support your regulatory and research requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Reconstituted solutions should be aliquoted and stored at -70°C to -80°C to prevent repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure containers are sealed under anhydrous conditions after each use.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/MS)	Conforms to standard
Purity (SDS-PAGE)	≥ 95%
Biological Activity (Chromogenic Assay)	≥ 90% of stated activity
Protein Content (UV Absorbance)	≥ 90% of label claim
Endotoxin (LAL Test)	< 1.0 EU/mg
Moisture Content (Karl Fischer)	< 3.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.