Description

Prothrombin (1-45) CAS NO 122168-64-3 is a defined peptide fragment derived from the N-terminal region of human prothrombin, a key zymogen in the blood coagulation cascade. This high-purity synthetic peptide is critical for advanced biochemical research and diagnostic development, serving as a precise tool for studying enzyme activation mechanisms and substrate specificity. It is primarily utilized by research institutions, pharmaceutical R&D departments, and in vitro diagnostic (IVD) manufacturers focused on hemostasis and thrombosis.

Application

• Biochemical Research: Used as a specific substrate or inhibitor in enzymatic studies to investigate the kinetics and regulation of thrombin generation and activity.
• Diagnostic Reagent Development: Serves as a critical component in the formulation of calibrators, controls, and assay kits for measuring prothrombin time (PT) and other coagulation factors.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) assays to identify and characterize novel anticoagulant or procoagulant drug candidates.
• Structure-Activity Relationship (SAR) Studies: Provides a well-defined molecular template for studying the interaction between prothrombin fragments and their binding partners, antibodies, or receptors.
• Antibody Production & Epitope Mapping: Acts as an immunogen or target antigen for generating and characterizing monoclonal and polyclonal antibodies specific to the prothrombin sequence.
• Quality Control Reference Standard: Used as an analytical standard in quality control laboratories to ensure the consistency and performance of therapeutic proteins or diagnostic assays related to blood coagulation.

Basic Information

Product Name	Prothrombin (1-45)
CAS No.	122168-64-3
Molecular Formula	C209H338N62O65S2
Molecular Weight	~4760.4 g/mol
Synonyms	Prothrombin Fragment 1-45; Human Prothrombin (1-45); Prothrombin 1-45 Peptide; Gla-domain peptide of prothrombin; Coagulation Factor II (1-45); FII (1-45); PT (1-45); N-terminal Fragment of Prothrombin
EINECS	Contact for details

Quality Control

Our Prothrombin (1-45) is manufactured under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and mass spectrometry (MS) for identity confirmation. We provide a detailed Certificate of Analysis (COA) with each shipment, documenting purity, peptide content, and other critical parameters. Our quality system is designed to support research and diagnostic applications requiring reliable and traceable materials.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to store desiccated. Allow the vial to warm to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC & MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Peptide Content	≥ 80% (by weight)
Amino Acid Sequence	H2N-ANRY...-COOH (Human Prothrombin 1-45)
Single Impurity (HPLC)	≤ 1.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water or buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.