Description

Bsm I CAS NO 122007-72-1 is a high-purity restriction enzyme, a critical tool for molecular biology research and biotechnological applications. This enzyme enables precise DNA cleavage at specific recognition sequences, which is fundamental for genetic engineering, cloning, and genomic analysis. It is an essential reagent for pharmaceutical R&D, academic research institutions, and diagnostic kit manufacturers requiring reliable and specific DNA manipulation.

Application

• Molecular Cloning: Essential for the precise insertion of DNA fragments into vectors during recombinant DNA technology workflows.
• Genetic Engineering: Used to construct genetically modified organisms (GMOs) and perform gene editing in foundational research.
• Genomic DNA Mapping: Facilitates the analysis of genome structure and the identification of genetic variations through restriction fragment length polymorphism (RFLP).
• Diagnostic Assay Development: Employed in the design and production of diagnostic tests that rely on specific DNA sequence detection.
• Pharmaceutical Research: Supports drug discovery and development, particularly in the characterization of plasmid DNA and the production of biologics.
• Academic & Industrial R&D: A staple reagent in laboratories for teaching purposes and advanced research in genetics, microbiology, and synthetic biology.

Basic Information

Product Name	Bsm I
CAS No.	122007-72-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	BsmI Restriction Endonuclease; Endodeoxyribonuclease BsmI; Restriction Enzyme Bsm I; BsmI (Bacillus stearothermophilus); Type II Restriction Enzyme BsmI; EC 3.1.21.4 (associated activity)
EINECS	Contact for details

Quality Control

Our Bsm I is produced under stringent quality management systems to ensure batch-to-batch consistency and optimal enzymatic activity. Each lot undergoes rigorous testing for purity, specific activity, and the absence of contaminating nucleases. A comprehensive Certificate of Analysis (COA) detailing assay results, storage buffer composition, and unit definition is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C. Avoid repeated freeze-thaw cycles. The enzyme is supplied in a stabilized storage buffer; please handle on ice when in use.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cleaves DNA at the specific recognition sequence
Assay (Activity)	≥ 10,000 units/mL (or as per COA)
Purity (SDS-PAGE)	≥ 95%
Contaminating Nuclease Activity	Not detected
Storage Buffer	Tris-HCl, KCl, DTT, Glycerol, EDTA (pH 7.5 at 25°C)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.