Description

8-Azido-2'-o-Dansyladenosine Triphosphate is a chemically modified nucleotide analog that serves as a critical tool in advanced biochemical research. This compound is specifically engineered to act as a photoaffinity labeling agent and a fluorescent probe, enabling the study of nucleotide-binding proteins and enzymatic mechanisms. It is an essential reagent for researchers in structural biology, enzymology, and drug discovery, particularly for investigating ATP-dependent processes through cross-linking and fluorescence-based detection methods.

Application

• Photoaffinity Labeling: Used to covalently tag and identify ATP-binding sites on proteins and enzymes upon UV irradiation.
• Fluorescence-Based Assays: Serves as a dansyl fluorophore-labeled substrate for real-time monitoring of kinase, polymerase, and ATPase activity.
• Structural Biology Studies: Aids in mapping active sites and conformational changes in nucleotide-binding proteins via cross-linking experiments.
• Enzyme Mechanism Analysis: Employed to probe the catalytic cycles and inhibitor interactions of ATP-utilizing enzymes.
• Bioconjugation & Probe Development: Acts as a versatile building block for creating advanced diagnostic and research probes through click chemistry (via the azido group).
• Drug Discovery Screening: Utilized in high-throughput screening assays to identify novel modulators of ATP-dependent targets.

Basic Information

Product Name	8-Azido-2'-o-Dansyladenosine Triphosphate
CAS No.	121258-46-6
Molecular Formula	C₂₇H₃₁N₁₀O₁₃P₃
Molecular Weight	844.55 g/mol
Synonyms	8-N₃-2'-O-Dansyl-ATP; 8-Azido-2'-O-(5-dimethylaminonaphthalene-1-sulfonyl)adenosine 5'-triphosphate; 8-Azido-2'-O-dansyl-ATP; Dansyl-8-azido-ATP; 8-Azido-2'-O-(dansyl)adenosine triphosphate; Photoaffinity ATP analog, dansyl-labeled; N⁶-(Dimethylamino)naphthalenesulfonyl-8-azido-ATP
EINECS	Contact for details

Quality Control

Our 8-Azido-2'-o-Dansyladenosine Triphosphate is manufactured under stringent conditions to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each lot undergoes comprehensive analytical characterization, including HPLC purity analysis, mass spectrometry (MS) confirmation, and functional activity validation in standard assay formats. A detailed Certificate of Analysis (COA) documenting identity, purity, and stability is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere and avoid repeated freeze-thaw cycles. Keep the container sealed and allow it to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid or lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Functional Activity	Conforms in standard enzymatic assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.