Description

Polyubiquitin is a polymeric chain of the small regulatory protein ubiquitin, linked through isopeptide bonds, primarily via lysine residues. This compound is essential for studying and modulating the ubiquitin-proteasome system, a critical pathway for targeted protein degradation and cellular regulation. It serves as a vital research tool for scientists in biochemistry, molecular biology, and drug discovery, particularly for investigating protein turnover, signal transduction, and disease mechanisms.

Application

• Biochemical Research: Used as a substrate or standard in in vitro assays to study ubiquitination enzymes (E1, E2, E3 ligases) and deubiquitinating enzymes (DUBs).
• Proteasome Function Studies: Essential for investigating the recognition and degradation of polyubiquitinated proteins by the 26S proteasome complex.
• Drug Discovery & Screening: Serves as a key reagent in high-throughput screening campaigns to identify novel inhibitors or activators of the ubiquitin-proteasome pathway for therapeutic development.
• Antibody & Assay Development: Used to generate and validate antibodies specific to polyubiquitin chains or as a coating antigen in ELISA and other immunoassays.
• Structural Biology: Provides material for X-ray crystallography, NMR, and cryo-EM studies to elucidate the structure of ubiquitin chains and their complexes.
• Cell Signaling Research: Employed to understand the role of specific polyubiquitin chain linkages (e.g., K48, K63) in cellular processes like DNA repair, inflammation, and autophagy.

Basic Information

Product Name	Polyubiquitin
CAS No.	120904-94-1
Molecular Formula	(C378H630N105O118S1)n (Ubiquitin monomer)
Molecular Weight	~8.6 kDa (Monomer); Polymer MW varies by chain length
Synonyms	Ubiquitin Polymer; Poly-Ubiquitin; Multi-Ubiquitin Chain; Ubiquitin Conjugate; Ubiquitin Chain; Ubiquitin Oligomer; Ubn; ISG15 (Interferon-stimulated gene 15) - related ubiquitin-like modifier
EINECS	Contact for details

Quality Control

Our Polyubiquitin is produced and tested under stringent conditions to ensure high purity, consistent chain length, and biological activity. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing specifications for identity, purity, and functional activity. We adhere to ISO 9001 quality management principles, and our processes are designed to support research requiring reliable and reproducible biochemical reagents.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Avoid repeated freeze-thaw cycles. Aliquot upon receipt for optimal preservation.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (SDS-PAGE & Western Blot)	Conforms
Purity (SDS-PAGE)	≥ 90%
Protein Content (BCA)	≥ 80%
Endotoxin (LAL)	< 1.0 EU/μg
Biological Activity (In vitro Assay)	Conforms to reference standard
Solubility	Soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.