Description

2-Chloro-4-Nitrophenyl-β-D-Gluco- Pyranoside is a specialized chromogenic substrate designed for the sensitive detection and quantification of β-glucosidase enzyme activity. This compound matters because it provides a reliable, colorimetric readout critical for enzymatic assays in research, diagnostics, and quality control processes. It is primarily needed by professionals in the biotechnology, pharmaceutical, and diagnostic reagent manufacturing industries for applications in enzyme kinetics, high-throughput screening, and biochemical research.

Application

• Enzymatic Assay Substrate: A key chromogenic reagent for the specific detection and kinetic study of β-glucosidase enzymes.
• Diagnostic Reagent Manufacturing: Used in the formulation of diagnostic kits for the detection of enzymatic activity related to certain genetic disorders or microbial identification.
• Biochemical Research: Employed in laboratory research to investigate enzyme function, inhibition, and pathway analysis.
• Pharmaceutical Screening: Utilized in high-throughput screening (HTS) platforms to identify potential drug candidates that modulate β-glucosidase activity.
• Quality Control in Biotechnology: Serves as a standard reagent for quality assurance testing of enzyme preparations and related bioproducts.
• Microbiology Studies: Applied in microbial assays to detect β-glucosidase activity as a phenotypic marker for certain bacteria and fungi.

Basic Information

Product Name	2-Chloro-4-Nitrophenyl-β-D-Gluco- Pyranoside
CAS No.	120221-14-9
Molecular Formula	C12H14ClNO8
Molecular Weight	335.69 g/mol
Synonyms	2-Chloro-4-nitrophenyl β-D-glucopyranoside; 4-Nitro-2-chlorophenyl β-D-glucopyranoside; β-Glucosidase Substrate (chromogenic); CNP-β-Glucoside; 2-Chloro-4-nitrophenyl-β-D-glucoside; p-Nitrophenyl-2-chloro-β-D-glucopyranoside; Chromogenic β-Glucoside Substrate
EINECS	Contact for details

Quality Control

Our 2-Chloro-4-Nitrophenyl-β-D-Gluco- Pyranoside is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive analytical applications. Each lot undergoes rigorous testing, including HPLC purity analysis, identity confirmation (IR/MP), and spectrophotometric assessment to meet precise specifications. Certificates of Analysis (COA) detailing all quality parameters are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (typically 15-25°C). This product is light-sensitive and should be handled accordingly to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Water Content (KF)	≤ 1.0%
Absorbance Spectrum	Conforms to reference
Solubility	Soluble in DMSO, Methanol; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.