Description

Collagen Type IV Alpha1 (531-543) is a specific, high-purity synthetic peptide fragment derived from the α-1 chain of Type IV collagen. This compound is critical for advanced biomedical research and development, particularly in the study of basement membrane structure, cell adhesion, and angiogenesis. It serves as an essential tool for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors who require precise molecular probes for immunological assays, epitope mapping, and therapeutic target validation.

Application

• Biomedical Research: Used as a specific antigen for studying autoimmune diseases, particularly Goodpasture's syndrome, where antibodies target the α-3 chain of Type IV collagen.
• Immunoassay Development: Serves as a coating antigen or standard in ELISA (Enzyme-Linked Immunosorbent Assay) and other immunoassays to detect and quantify specific autoantibodies.
• Epitope Mapping & Characterization: A key reagent for defining the precise binding sites (epitopes) of monoclonal and polyclonal antibodies directed against Type IV collagen.
• Vaccine Research: Investigated in the development of tolerogenic vaccines for autoimmune conditions targeting basement membrane components.
• Cell Adhesion Studies: Utilized in in vitro studies to understand the role of specific collagen domains in cell-matrix interactions and signaling.
• Quality Control & Standardization: Acts as a reference standard for the quality control of diagnostic kits and reagents targeting anti-collagen antibodies.

Basic Information

Product Name	Collagen Type IV Alpha1 (531-543)
CAS No.	119953-02-5
Molecular Formula	C62H98N20O21
Molecular Weight	1427.6 g/mol
Synonyms	Type IV Collagen α-1 Chain Fragment (531-543); Collagen IV α1(531-543); Goodpasture Antigen-Related Peptide; GP-Antigen Peptide; α1(IV)NC1 [531-543]; H-Gly-Val-Gln-Pro-Gly-Ile-Leu-Gly-Gln-Lys-Pro-Gly-Glu-OH; GVQPGILGQKPGE; (Alpha1)IV 531-543; NC1 Domain Peptide (α1 chain)
EINECS	Contact for details

Quality Control

Our Collagen Type IV Alpha1 (531-543) is manufactured under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes comprehensive analytical characterization, including HPLC for purity verification, mass spectrometry (MS) for identity confirmation, and amino acid analysis (AAA). Certificates of Analysis (COA) detailing all specifications and test results are provided to guarantee compliance with research-grade standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC & MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Amino Acid Composition	Conforms to theoretical values
Peptide Content	≥ 70% (by weight)
Solubility	Soluble in water or dilute acetic acid
Single Impurity (HPLC)	≤ 1.0%
Water Content (KF)	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.