Description

5''-o-(4,4''-Dimethoxytrityl)-Inosine is a key nucleoside derivative, specifically a protected inosine monomer, widely used in oligonucleotide synthesis. This compound is critical for ensuring high coupling efficiency and purity during automated solid-phase synthesis, particularly for antisense oligonucleotides and siRNA. It is an essential raw material for pharmaceutical R&D and production teams focused on nucleic acid therapeutics, diagnostics, and molecular biology research.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): As a phosphoramidite building block for the controlled assembly of DNA/RNA strands.
• Antisense Oligonucleotide (ASO) Manufacturing: Production of therapeutic oligonucleotides targeting specific mRNA sequences.
• siRNA and miRNA Synthesis: Key precursor in the manufacture of small interfering RNA and microRNA for gene silencing applications.
• Diagnostic Probe Development: Used in creating labeled probes for PCR, FISH, and other hybridization-based diagnostic assays.
• Research-Grade Nucleotide Chemistry: Serves as a versatile intermediate in academic and industrial research exploring novel nucleoside analogs.
• GMP-Grade Therapeutic Production: Suitable for use in cGMP manufacturing processes for clinical-stage and commercial nucleic acid drugs.

Basic Information

Product Name	5''-o-(4,4''-Dimethoxytrityl)-Inosine
CAS No.	119898-59-8
Molecular Formula	C31H30N4O7
Molecular Weight	570.59 g/mol
Synonyms	5'-O-DMT-Inosine; DMT-Inosine; 5'-O-(4,4'-Dimethoxytrityl)inosine; 9-[(2R,3R,4S,5R)-3,4-Dihydroxy-5-[[bis(4-methoxyphenyl)-phenylmethoxy]methyl]oxolan-2-yl]-3H-purin-6-one; Inosine, 5'-O-(4,4'-dimethoxytrityl)-; N6-Hydroxy-5'-O-DMT-adenosine (common misnomer); DMT-I (abbreviation); Protected Inosine Monomer
EINECS	Contact for details

Quality Control

Our 5''-o-(4,4''-Dimethoxytrityl)-Inosine is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (typically ≥98%), to ensure it meets the stringent requirements for oligonucleotide synthesis. Certificates of Analysis (COA) with full chromatographic data are provided for every shipment. We support development under GMP guidelines for advanced therapeutic applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere (e.g., nitrogen or argon) for long-term stability. For optimal performance, desiccate at room temperature before use in sensitive synthetic applications.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, pyridine

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.