Description

3-Indoxyl-β-D-Glucuronic Acid, Sodium Salt is a high-purity biochemical reagent widely used as a critical substrate for enzymatic detection. This compound is essential for the specific and sensitive colorimetric or fluorometric detection of β-glucuronidase activity. It is primarily utilized by research institutions, diagnostic kit manufacturers, and quality control laboratories in the life sciences and pharmaceutical sectors. The product offers reliable performance for applications requiring precise enzymatic analysis.

Application

• Enzymatic Substrate for β-Glucuronidase: Primary substrate in assays to detect and quantify β-glucuronidase enzyme activity in research and diagnostics.
• Microbiological Testing: Used in culture media and diagnostic kits for the identification of Escherichia coli and other β-glucuronidase-positive microorganisms.
• Molecular Biology & Cell Biology Research: Employed in reporter gene assays (e.g., GUS gene) for studying gene expression and promoter activity in plant and microbial systems.
• Diagnostic Reagent Manufacturing: A key raw material in the production of clinical and veterinary diagnostic test strips, kits, and automated analyzer reagents.
• Food & Water Safety Analysis: Applied in rapid methods for the detection of fecal coliforms and E. coli contamination in food, beverage, and water samples.
• Pharmaceutical Quality Control: Used in enzymatic purity tests and stability-indicating assays for drug products and raw materials.

Basic Information

Product Name	3-Indoxyl-β-D-Glucuronic Acid, Sodium Salt
CAS No.	119736-51-5
Molecular Formula	C14H14NNaO8
Molecular Weight	347.25 g/mol
Synonyms	5-Bromo-4-chloro-3-indolyl-β-D-glucuronic acid sodium salt; X-Glucuronide sodium salt; 3-Indoxyl-β-D-glucopyranosiduronic acid sodium salt; Sodium 3-indoxyl-β-D-glucuronate; BCIG; X-GlcA; GUS substrate; β-Glucuronidase substrate
EINECS	Contact for details

Quality Control

Our 3-Indoxyl-β-D-Glucuronic Acid, Sodium Salt is manufactured under a strict quality management system. Each batch undergoes comprehensive analytical testing to ensure identity, purity, and performance consistency suitable for sensitive diagnostic and research applications. Certificates of Analysis (COA) documenting purity (typically >95% by HPLC), spectral identification, and impurity profiles are provided with every shipment. We support compliance with relevant industry standards for reagent-grade chemicals.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to reach room temperature before opening to minimize moisture condensation. For long-term stability, store desiccated at -20°C.

Specification

Item	Specification
Appearance	Off-white to light yellow powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Freely soluble in water, DMSO
Enzymatic Activity	Produces characteristic blue precipitate with β-glucuronidase

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.