Description

2-Hydroxybiphenyl 3-Monooxygenase Recomb is a recombinant enzyme, a key biocatalyst in the oxidative metabolism of aromatic compounds. This engineered enzyme offers high specificity and activity for the regioselective hydroxylation of biphenyl derivatives, making it a valuable tool for synthetic biology and green chemistry applications. It is primarily utilized by researchers and process developers in the pharmaceutical, agrochemical, and environmental biotechnology sectors for the synthesis of fine chemicals and bioremediation studies.

Application

• Biocatalysis for Fine Chemical Synthesis: Used in the regioselective hydroxylation of biphenyl and related aromatic scaffolds to produce pharmaceutical intermediates and specialty chemicals.
• Metabolic Pathway Engineering: A critical component in constructing synthetic microbial pathways for the production of high-value compounds from aromatic feedstocks.
• Environmental Bioremediation Research: Employed in studies aimed at degrading persistent aromatic pollutants, such as certain biphenyls, due to its monooxygenase activity.
• Enzyme Mechanism & Kinetics Studies: Serves as a model enzyme in biochemical research to understand structure-function relationships in cytochrome P450-type monooxygenases.
• Biosensor Development: Potential use in the development of enzymatic sensors for detecting specific aromatic compounds in environmental or industrial samples.

Basic Information

Product Name	2-Hydroxybiphenyl 3-Monooxygenase Recomb
CAS No.	118251-39-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	2-Hydroxybiphenyl 3-Monooxygenase (Recombinant); Biphenyl-2-ol 3-Monooxygenase; HbpA; Recombinant 2-Hydroxybiphenyl 3-Hydroxylase; EC 1.14.14.-; 2,3-Dihydroxybiphenyl Dioxygenase (related activity); Aromatic Ring-Hydroxylating Dioxygenase
EINECS	Contact for details

Quality Control

Our recombinant enzyme products are manufactured under stringent quality control protocols to ensure batch-to-batch consistency, high specific activity, and purity. Each lot is tested for protein concentration, enzymatic activity, and the absence of contaminating protease activities. A comprehensive Certificate of Analysis (COA) detailing purity (by SDS-PAGE), specific activity, buffer composition, and storage conditions is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below. Avoid repeated freeze-thaw cycles. This material is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after use to prevent moisture absorption.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Purity (SDS-PAGE)	≥ 90%
Protein Concentration	Specified on COA
Specific Activity	≥ [Value] U/mg (Contact for details)
Buffer	Tris-HCl or Phosphate buffer, pH ~7.5
Stabilizers	Glycerol, DTT (as specified)
Endotoxin Level	< 1.0 EU/µg (if applicable)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.