Description

Nucleophosmin is a multifunctional nucleolar phosphoprotein that plays a critical role in ribosome biogenesis, cell cycle progression, and centrosome duplication. Its significance extends to its function as a molecular chaperone for both proteins and nucleic acids, making it a protein of high interest in fundamental biological research. This product is essential for researchers and developers in the fields of oncology, molecular biology, and cell signaling studies, particularly for investigating its role in cancer pathogenesis and as a potential diagnostic marker.

Application

• Biomedical Research: Used as a key reagent in studies of nucleolar function, cell proliferation, and apoptosis.
• Cancer Research: Critical for investigating its role in leukemogenesis (particularly in acute myeloid leukemia with NPM1 mutations) and solid tumors.
• Diagnostic Development: Serves as an antigen or standard in the development of immunoassays and immunohistochemistry tests for cancer diagnosis.
• Protein-Protein Interaction Studies: Utilized in pull-down assays, co-immunoprecipitation, and yeast two-hybrid screens to map interaction networks.
• Drug Discovery & Target Validation: Employed in high-throughput screening platforms to identify compounds that modulate NPM1 function or localization.
• Cell Biology Assays: Used in microscopy and cellular fractionation experiments to study nucleolar-cytoplasmic shuttling.

Basic Information

Product Name	Nucleophosmin
CAS No.	117896-08-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	NPM1; Nucleolar phosphoprotein B23; Numatrin; NO38; Nucleophosmin 1; B23; NPM; Nucleolar protein NO38
EINECS	Contact for details

Quality Control

Our Nucleophosmin is produced and handled under stringent quality management protocols. Each batch undergoes comprehensive analytical testing, including purity assessment by electrophoresis and functional activity validation, to ensure consistency and biological relevance for research applications. Certificates of Analysis (COA) detailing specific purity, concentration, and bioactivity data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to reach room temperature before opening to minimize moisture absorption. For extended storage, consider aliquoting to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or solution
Identification (SDS-PAGE / Western Blot)	Corresponds to Nucleophosmin (B23)
Purity (SDS-PAGE)	> 90%
Protein Concentration	Provided on COA
Biological Activity	Assessed by defined binding or functional assay
Endotoxin Level	< 1.0 EU/µg (for cell culture grades)
Solubility	Soluble in appropriate aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.