Description

Wingless Protein, Drosophila is a highly purified recombinant protein derived from the *Drosophila melanogaster* (fruit fly) model organism, specifically the Wingless (Wg) signaling molecule. This protein is a critical reagent for fundamental research in developmental biology, cell signaling, and oncology, serving as a key ligand in the evolutionarily conserved Wnt signaling pathway. It is essential for scientists and R&D teams in academic, pharmaceutical, and biotechnology sectors studying cell fate determination, tissue regeneration, and disease mechanisms.

Application

• Cell Signaling & Developmental Biology Research: Used as a standard ligand to activate the canonical Wnt/β-catenin signaling pathway in *in vitro* cell-based assays.
• Stem Cell Research: Employed to maintain stem cell pluripotency and direct differentiation in various progenitor cell cultures.
• Drug Discovery & Screening: Serves as a critical tool in high-throughput screening (HTS) platforms to identify novel agonists or antagonists of the Wnt pathway for therapeutic development.
• Protein-Protein Interaction Studies: Utilized in pull-down assays, co-immunoprecipitation, and surface plasmon resonance (SPR) to map interactions with receptors like Frizzled and co-receptors.
• Biomarker & Diagnostic Development: Acts as a reference standard in the development of immunoassays (ELISA, Western Blot) to detect pathway activity in clinical samples.
• Gene Expression Analysis: Used to treat cells to study downstream transcriptional changes via qPCR or RNA-seq, elucidating target genes of the Wnt pathway.

Basic Information

Product Name	Wingless Protein, Drosophila
CAS No.	117758-26-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Wingless Protein (Drosophila); Wg Protein; Drosophila Wingless Signaling Protein; Recombinant Drosophila Wingless; Wnt-1 Homolog (Drosophila); Wingless-type MMTV integration site family, Drosophila homolog; Wnt Ligand
EINECS	Contact for details

Quality Control

Our Wingless Protein, Drosophila is produced under stringent conditions to ensure high biological activity and batch-to-batch consistency. Each lot undergoes comprehensive analytical testing, including purity assessment, endotoxin level determination, and functional activity verification in cell-based assays. A Certificate of Analysis (COA) detailing purity, concentration, biological activity, and sterility (where applicable) is provided with every shipment to support your research compliance and reproducibility requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at ≤ -20°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. Aliquot upon receipt for single-use to maintain optimal activity. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use.

Specification

Item	Specification
Appearance	White or off-white lyophilized powder or clear solution
Purity (SDS-PAGE)	≥ 95%
Endotoxin Level	< 1.0 EU/μg
Biological Activity (Cell-based assay)	Determined per batch; reported on COA
Protein Concentration	As specified on label and COA
Buffer Composition	Specified on COA
Sterility (for aseptic grade)	Sterile-filtered, 0.2 μm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.