Description

n-Succinyl-Ala-Phe-Lys 7-Amido-4-Methylcoumarin Acetate Salt is a specialized fluorogenic peptide substrate designed for the sensitive detection and quantification of protease activity. This compound matters because it provides a reliable, high-throughput method for enzymatic assays, crucial for research and quality control in life sciences. It is primarily needed by pharmaceutical R&D teams, diagnostic kit manufacturers, and academic researchers focused on enzymology, drug discovery, and biochemical pathway analysis.

Application

• Protease Activity Assays: As a fluorogenic substrate for trypsin, plasmin, and related serine proteases in kinetic studies.
• High-Throughput Screening (HTS): For drug discovery platforms targeting protease inhibitors in pharmaceutical development.
• Diagnostic Development: Used in the formulation of clinical diagnostic tests for conditions involving abnormal protease levels.
• Biochemical Research: A key tool for studying enzyme kinetics, specificity, and inhibition mechanisms in academic and industrial laboratories.
• Quality Control: Employed in biomanufacturing to monitor and validate the activity of protease-containing products or to ensure their absence.

Basic Information

Product Name	n-Succinyl-Ala-Phe-Lys 7-Amido-4-Methylcoumarin Acetate Salt
CAS No.	117756-27-1
Molecular Formula	C33H41N5O9 • xC2H4O2
Molecular Weight	651.71 g/mol (Free base)
Synonyms	Suc-AFK-AMC; Succinyl-Alanine-Phenylalanine-Lysine-7-Amido-4-Methylcoumarin; Suc-Ala-Phe-Lys-AMC; AMC Substrate Suc-AFK; Fluorogenic Substrate for Trypsin; CAS 117756-27-1; N-Succinyl-Ala-Phe-Lys-AMC Acetate
EINECS	Contact for details

Quality Control

Our n-Succinyl-Ala-Phe-Lys 7-Amido-4-Methylcoumarin Acetate Salt is produced and tested under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity verification, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee batch-to-batch consistency and performance reliability.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Conforms (Enzymatic Assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.