Description

Lens Culinaris Agglutinin CAS NO 117675-52-2 is a highly purified lectin protein isolated from lentils (*Lens culinaris*). This compound is valued for its specific carbohydrate-binding properties, enabling selective recognition and interaction with glycoproteins and cell surface sugars. It serves as a critical research and diagnostic tool for scientists and manufacturers in the life sciences, biotechnology, and pharmaceutical development sectors.

Application

• Biomedical Research: Used as a probe in glycobiology to study cell surface glycosylation patterns, cell-cell interactions, and protein-carbohydrate binding specificity.
• Diagnostic Assays: Employed in lectin-based assays, histochemistry, and blotting techniques for the detection and characterization of glycoproteins and polysaccharides.
• Cell Separation & Purification: Facilitates the isolation of specific cell populations (e.g., stem cells, lymphocytes) through affinity chromatography or magnetic bead separation based on surface glycan markers.
• Pharmaceutical Development: Acts as a tool for quality control in the analysis of glycosylated biopharmaceuticals, such as monoclonal antibodies and recombinant proteins.
• Food Safety & Analysis: Applied in lectin-based sensors and tests for the detection of microbial contaminants or specific carbohydrate residues in food products.
• Mitogenic Studies: Used to stimulate lymphocyte proliferation in immunology research to study immune cell activation and response.

Basic Information

Product Name	Lens Culinaris Agglutinin
CAS No.	117675-52-2
Molecular Formula	Contact for details
Molecular Weight	~49 kDa (Tetramer)
Synonyms	Lentil Lectin; LCA; *Lens culinaris* Hemagglutinin; *Lens culinaris* Lectin; LcH; LcL; *Lens esculenta* Lectin; *Lens culinaris* Agglutinin (LCA)
EINECS	Contact for details

Quality Control

Our Lens Culinaris Agglutinin is produced under stringent conditions to ensure high biological activity and purity, suitable for research and diagnostic applications. Each batch is subjected to rigorous quality testing, including analysis for protein content, hemagglutination activity, and specificity. A comprehensive Certificate of Analysis (COA) detailing purity, activity, and absence of contaminating proteases is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, consider lyophilized powder kept at -20°C. Avoid repeated freeze-thaw cycles of solutions.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR / Electrophoresis)	Conforms to standard
Protein Content (Biuret / UV)	> 95%
Hemagglutination Activity	> 128 HAU/mg (vs. Rabbit Erythrocytes)
Specificity	Binds α-linked mannose, glucose, and N-acetylglucosamine
Solubility	Soluble in water, PBS, or TBS buffer
Endotoxin Level (LAL)	< 1.0 EU/mg
Protease Activity	Not detected

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.