Description

Amphiregulin Human Recombinant CAS NO 117147-70-3 is a high-purity, biologically active protein belonging to the epidermal growth factor (EGF) family. This recombinant growth factor is critical for research and development in cell biology and therapeutic discovery, offering consistent quality and reliable performance. It is essential for scientists and manufacturers in the pharmaceutical, biotechnology, and life science research sectors who require a validated reagent for studying epithelial cell proliferation, wound healing, and cancer biology.

Application

• Cell Culture & Proliferation Studies: Used as a potent mitogen to stimulate the growth of various epithelial and fibroblast cell lines in vitro.
• Cancer Research: Investigated for its role in tumorigenesis, metastasis, and as a potential biomarker or therapeutic target in oncology research.
• Wound Healing Models: Applied in research models to study the mechanisms of tissue repair, regeneration, and keratinocyte migration.
• Signal Transduction Pathways: Serves as a key ligand to activate the EGFR/ErbB signaling pathway for downstream biochemical analysis.
• Biopharmaceutical Development: Employed in assay development, potency testing, and as a reference standard for quality control of related biologic products.
• Differentiation Studies: Used to induce or modulate the differentiation of specific stem cell or progenitor cell populations.

Basic Information

Product Name	Amphiregulin Human Recombinant
CAS No.	117147-70-3
Molecular Formula	C860H1342N232O252S10
Molecular Weight	~19.9 kDa
Synonyms	Amphiregulin; AREG; Colorectum cell-derived growth factor; CRDGF; Schwannoma-derived growth factor; SDGF; Amphiregulin precursor; AR; Amphiregulin (Human); Recombinant Human Amphiregulin
EINECS	Contact for details

Quality Control

Our Amphiregulin Human Recombinant is manufactured under stringent conditions to ensure the highest standards of purity and biological activity. Each batch is rigorously tested for identity, purity, potency, and endotoxin levels. Certificates of Analysis (COA) detailing specifications such as purity by SDS-PAGE and HPLC, biological activity via cell-based assays, and endotoxin content are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term storage, keep lyophilized material at -20°C or below. Reconstituted protein aliquots should be stored at -70°C to -80°C and avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the vial is at room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White or off-white lyophilized powder
Purity (SDS-PAGE)	> 95%
Purity (HPLC)	> 98%
Endotoxin Level	< 1.0 EU/µg
Biological Activity	Determined by cell proliferation assay
Identification (Mass Spec)	Consistent with theoretical molecular weight
Solubility	Soluble in sterile PBS or cell culture medium

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.