Description

3'-Keto-2'-Deoxyuridine 5'-Triphosphate is a chemically modified nucleotide triphosphate analog of significant interest in biochemical research and development. This compound serves as a critical tool for studying enzyme mechanisms, particularly those involving DNA polymerases and reverse transcriptases, due to its altered sugar moiety. It is primarily utilized by research institutions, pharmaceutical R&D departments, and diagnostic kit manufacturers engaged in advanced molecular biology and antiviral research. Its specific structure allows for the investigation of nucleotide incorporation and chain termination events.

Application

• Biochemical Research: Used as a substrate analog to study the kinetics and fidelity of DNA polymerases, RNA polymerases, and reverse transcriptases.
• Enzyme Mechanism Studies: Essential for probing active sites and catalytic pathways of nucleotide-processing enzymes due to the reactive 3'-keto group.
• Antiviral Drug Development: Investigated as a potential chain terminator or inhibitor in the development of nucleoside analog-based therapeutics.
• Diagnostic Assay Development: Employed in the design and optimization of molecular diagnostic tests that require modified nucleotides for specific detection or amplification.
• Structural Biology: Used in X-ray crystallography and NMR studies to determine enzyme-ligand complex structures.
• Metabolic Pathway Analysis: Aids in tracing and understanding nucleotide salvage and anabolic pathways within cells.

Basic Information

Product Name	3'-Keto-2'-Deoxyuridine 5'-Triphosphate
CAS No.	117098-38-1
Molecular Formula	C10H14N2O15P3
Molecular Weight	510.14 g/mol
Synonyms	3'-Keto-dUTP; 2'-Deoxyuridine-5'-triphosphate, 3'-keto-; 3'-Oxo-2'-deoxyuridine 5'-triphosphate; Keto-dUTP; dU-3'-keto-TP; 3'-Keto-2'-deoxyuridine triphosphate; 3'-OxodUTP; 3'-Keto-deoxyuridine triphosphate
EINECS	Contact for details

Quality Control

Our 3'-Keto-2'-Deoxyuridine 5'-Triphosphate is produced under stringent quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity assessment and NMR for structural confirmation, to ensure it meets the exacting standards required for sensitive research applications. A detailed Certificate of Analysis (COA) providing batch-specific data on purity, identity, and impurity profile is supplied with every order.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Biological Activity	Tested in standard polymerase assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.