Description

2,3-Bis(2-Methoxy-4-Nitro-5-Sulfophenyl)-5-((Phenylamino)Carbonyl)-2H-Tetrazolium Hydroxide is a specialized tetrazolium salt widely recognized as a critical chromogenic substrate in biochemical and cell-based assays. Its primary value lies in its ability to produce a highly colored, water-soluble formazan dye upon reduction by cellular dehydrogenases, making it an indispensable tool for quantifying cell viability, proliferation, and cytotoxicity. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors, where precise and reliable metabolic activity measurements are paramount.

Application

• Cell Viability and Proliferation Assays: Used as the key substrate in MTT and related assays to measure mitochondrial activity in live cells.
• Cytotoxicity Screening: Employed in high-throughput screening (HTS) platforms to evaluate the toxic effects of drug candidates, chemicals, and nanomaterials.
• Microbiology Research: Applied in assays to study microbial cell growth, metabolic activity, and antibiotic susceptibility.
• Biochemical Enzyme Assays: Serves as an electron acceptor in dehydrogenase enzyme activity studies.
• Drug Discovery & Development: Integral to in vitro testing phases for assessing compound efficacy and safety profiles.
• Diagnostic Kit Manufacturing: A vital component in the formulation of commercial diagnostic kits for research and clinical use.

Basic Information

Product Name	2,3-Bis(2-Methoxy-4-Nitro-5-Sulfophenyl)-5-((Phenylamino)Carbonyl)-2H-Tetrazolium Hydroxide
CAS No.	117038-70-7
Molecular Formula	C29H23N7O12S2
Molecular Weight	725.66 g/mol
Synonyms	WST-1; Water Soluble Tetrazolium Salt 1; 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt (related compound); Tetrazolium Hydroxide, 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-; CCK-8 Assay Component; Cell Counting Kit-8 Reagent; MTS Analog; XTT Analog
EINECS	Contact for details

Quality Control

Every batch of 2,3-Bis(2-Methoxy-4-Nitro-5-Sulfophenyl)-5-((Phenylamino)Carbonyl)-2H-Tetrazolium Hydroxide is manufactured and tested under strict quality management systems to ensure the highest standards of purity and performance for sensitive bioassays. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) detailing purity, identity, and performance in standard assays are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure containers are sealed immediately after use to prevent degradation and moisture absorption. For long-term stability, consider storage under inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Absorbance (450 nm)	Meets performance criteria
Solubility	Freely soluble in water or buffer
Heavy Metals	< 10 ppm
Biological Performance	Validated in standard cell viability assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.