Description

4-(Trifluoromethyl)Umbelliferyl-β-D-Glucopyranoside is a specialized fluorogenic substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise detection and quantification of β-glucosidase activity. It is primarily utilized in the life sciences, pharmaceutical development, and industrial biotechnology sectors for applications ranging from enzyme kinetics studies to high-throughput screening.

Application

• Enzymatic Assay Substrate: A key fluorogenic probe for the detection and kinetic analysis of β-glucosidase enzymes.
• Diagnostic Reagent Manufacturing: Used in the formulation of diagnostic kits for genetic disorders like Gaucher disease, where β-glucosidase activity is a biomarker.
• Pharmaceutical Research & HTS: Employed in high-throughput screening (HTS) campaigns to identify modulators (inhibitors/activators) of β-glucosidase for drug discovery.
• Biochemical Research: Serves as a critical tool in fundamental enzymology studies to understand enzyme mechanism and specificity.
• Industrial Biocatalyst Monitoring: Applied in biofuel and biorefinery processes to monitor cellulase and other glycoside hydrolase activities.
• Microbiology: Used to detect β-glucosidase activity in microbial cultures for identification and metabolic profiling.

Basic Information

Product Name	4-(Trifluoromethyl)Umbelliferyl-β-D-Glucopyranoside
CAS No.	116981-86-3
Molecular Formula	C₁₆H₁₅F₃O₈
Molecular Weight	392.28 g/mol
Synonyms	4-Methylumbelliferyl-β-D-glucopyranoside, 4-Trifluoromethyl Derivative; 4-Trifluoromethylumbelliferyl β-D-Glucoside; 4-TFMUG; β-Glucosidase Substrate (Fluorogenic); 4-(Trifluoromethyl)-7-hydroxycoumarin β-D-glucopyranoside; 4-MU-β-Glc (Trifluoromethyl Analog); 7-Hydroxy-4-(trifluoromethyl)coumarin β-D-glucopyranoside
EINECS	Contact for details

Quality Control

Our 4-(Trifluoromethyl)Umbelliferyl-β-D-Glucopyranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance data. We adhere to relevant quality guidelines suitable for research and in-vitro diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. For long-term storage, consider storage at -20°C under desiccant. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Assay (HPLC)	≥ 98.0%
Water Content	≤ 1.0%
Fluorogenic Activity	Conforms to performance standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.